Liquid chromatographic determination of 4,4'-dinitrocarbanilide, the active component of the infertility agent nicarbazin, in chicken, duck, goose, and snake eggs.

Abstract

4,4'-Dinitrocarbanilide (DNC) was extracted from chicken, duck, goose, and snake eggs and isolated by reversed-phase liquid chromatography. DNC was detected by ultraviolet absorbance at 347 nm and quantitated by comparison with a calibration standard. Recoveries of DNC from fortified control chicken, duck, goose, and snake egg samples were determined for DNC levels of 0.16, 10, and 16 microg/g. The mean recoveries from chicken, duck, goose, and snake eggs were 92 +/- 4, 88 +/- 9, 87 +/- 7, and 95 +/- 6%, respectively. The method limits of detection for DNC in chicken, duck, goose, and snake eggs ranged from 0.015 to 0.035 microg/g. The reported method is much simpler than and equally efficient as previous methods developed for the determination of DNC residues in egg contents.

• Authors: Thomas M Primus, Dennis J Kohler, Margaret A Goodall, Christi Yoder, Thomas Mathies, Lowell Miller, John J Johnston, Kurt Vercauteren
• Journal: Journal of AOAC International (J AOAC Int) 2003 Nov-Dec Vol. 86 Issue 6 Pg. 1144-8 ISSN: 1060-3271 [Print] England
• PMID: 14979695 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carbanilides
• Fertility Agents
• Indicators and Reagents
• Solutions
• Nicarbazin
• 4,4'-dinitrocarbanilide

Topics

• Animals
• Calibration
• Carbanilides (analysis)
• Chickens
• Ducks
• Eggs (analysis)
• Fertility Agents (analysis)
• Geese
• Indicators and Reagents
• Nicarbazin (analysis)
• Reference Standards
• Snakes
• Solutions
• Spectrophotometry, Ultraviolet