Effects of a topical enamel matrix derivative on skin wound healing.

Abstract

Enamel matrix derivative, obtained from developing porcine teeth, is composed mainly of amelogenin proteins and used topically in periodontal surgery for advanced periodontitis to regenerate lost connective tissues. The primary objective of this study was to investigate the effects of enamel matrix derivative on skin wound healing. Secondly, in vitro effects of enamel matrix derivative on dermal fibroblasts and microvascular endothelial cells were examined. Full-thickness, circular 2-cm skin wounds in white 16-week-old rabbits were treated thrice weekly with enamel matrix derivative (30 mg/ml) in the vehicle propylene glycol alginate or with vehicle alone. Enamel matrix derivative treatment increased the amount of granulation tissue and accelerated time to complete epithelialization by 3 days (p < 0.001) compared to vehicle treatment. In cultured fibroblasts, vascular endothelial growth factor levels in conditioned media were increased more than fivefold (p < 0.001) with enamel matrix derivative treatment (0.1mg/ml) over control, measured by specific enzyme-linked immunosorbent assay. Enamel matrix derivative also increased release of matrix metalloproteinase-2 more than threefold from fibroblasts (p < 0.001) and from endothelial cells (p < 0.001). Thus, enamel matrix derivative significantly accelerated wound closure in rabbits, possibly by increasing levels of growth factors and proteinases important for granulation tissue formation and remodeling.

• Authors: Ursula Mirastschijski, Daniel Konrad, Eva Lundberg, Staale Petter Lyngstadaas, Lars N Jorgensen, Magnus S Agren
• Journal: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (Wound Repair Regen) 2004 Jan-Feb Vol. 12 Issue 1 Pg. 100-8 ISSN: 1067-1927 [Print] United States
• PMID: 14974971 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dental Enamel Proteins
• Growth Substances
• Vascular Endothelial Growth Factor A
• enamel matrix proteins
• Matrix Metalloproteinase 2

Topics

• Administration, Topical
• Animals
• Cells, Cultured
• Dental Enamel Proteins (administration & dosage)
• Endothelial Cells (drug effects, metabolism)
• Female
• Fibroblasts (drug effects, metabolism)
• Granulation Tissue (drug effects, metabolism)
• Growth Substances (administration & dosage)
• Male
• Matrix Metalloproteinase 2 (biosynthesis)
• Rabbits
• Skin (drug effects)
• Vascular Endothelial Growth Factor A (biosynthesis)
• Wound Healing (drug effects)