[Studies on the biotransformation of escin Ia by human intestinal bacteria and the anti-tumor activities of desacylescin I].

Abstract

OBJECTIVE:

To study Biotransformation of escin Ia by the crude enzymes of human intestinal bacteria and Lactobacillus brevis, determine the structures of biotransformation products and assay the inhibitory effect of desacylescin I on the tumor cell growth.

METHODS:

The escin Ia was incubated with crude enzymes of human intestinal bacteria and Lactobacillus brevis in vitro, respectively. The biotransformation products were isolated and purified by the chromatographic methods and the structures were determined by the spectroscopic techniques.

RESULTS:

Escin Ia was converted into isoescin Ia, desacylescin I, 21beta-O-tigloylprotoaescigenin and protoaescigenin by crude enzymes of human intestinal bacteria and Lactobacillus brevis. Desacylescin I showed potentially inhibitory effects on tumor cell growth of mouse sarcoma-180, hepatic carcinoma H(22) and lung carcinoma in vivo.

CONCLUSION:

The results suggest that Escin Ia was a prodrug and its structure can be converted by human intestinal bacteria and Lactobacillus brevis. Desacylescin I as a biotransformation product showed potentially inhibitory effects on mouse tumor, and a potential candidate for anti tumor agents.

• Authors: Xiu-Wei Yang, Jing Zhao, Jing-Rong Cui, Wei Guo
• Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (Beijing Da Xue Xue Bao Yi Xue Ban) Vol. 36 Issue 1 Pg. 31-5 (Feb 2004) ISSN: 1671-167X [Print] China
• PMID: 14970884 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Escin
• escin Ia

Topics

• Animals
• Antineoplastic Agents (pharmacokinetics, pharmacology)
• Bacteria (metabolism)
• Biotransformation
• Escin (pharmacokinetics, pharmacology)
• Female
• Humans
• Intestines (microbiology)
• Male
• Mice
• Sarcoma 180 (drug therapy)