Resveratrol, a red wine constituent polyphenol, protects from ischemia-reperfusion damage of the ovaries.

Abstract

OBJECTIVE:

The aim of this study is to investigate the effects of resveratrol on histopathological changes, antioxidant status and lipid peroxidation, in torsion-detorsion injury in rat ovaries.

METHOD:

To determine whether ischemia followed by reperfusion can induce ovarian oxidative damage, we created a model of adnexal ischemia-reperfusion by using rats. Ischemia was induced by unilateral occlusion of the tubo-ovarian vessels for 3 h. Reperfusion was achieved by releasing the occlusion and restoring the circulation for 3 h. Thirty-two adult female albino rats were divided equally into 4 groups: sham operation, torsion, saline/detorsion and resveratrol/detorsion. Rats in the torsion group were killed after 360 degrees clockwise adnexal torsion for 3 h. Resveratrol was injected intraperitoneally 30 min before detorsion in the resveratrol/detorsion group, and saline was administered in the saline/detorsion group. After 3 h of adnexal detorsion in both of these groups, the rats were killed and adnexa were removed. The tissue levels of malondialdehyde, reduced glutathione and xanthine oxidase activity were measured.

RESULTS:

Malondialdehyde and xanthine oxidase levels in the saline/detorsion group were increased significantly when compared to the torsion and sham operation groups (p < 0.001). Malondialdehyde levels in the resveratrol group were lower than in the saline/detorsion group, and differences between the two groups were statistically significant (p < 0.001). Xanthine oxidase levels in the resveratrol group were lower than in the saline/detorsion and torsion groups, and differences between these groups were statistically significant (p < 0.001). Reduced glutathione levels in the saline/detorsion group were decreased significantly when compared to the torsion and sham operation groups. Reduced glutathione levels in the resveratrol group were significantly higher than in the saline/detorsion group (p < 0.006). Histological examination showed a significant improvement in ovarian morphology in the resveratrol-treated rats compared with the ischemia and ischemia-reperfusion groups.

CONCLUSION:

Our results demonstrated that intraperitoneal resveratrol administration reduced the lipid peroxidation products of ischemic rats and ovarian damage was reduced as indicated by histological examination.

• Authors: Seyma Hascalik, Onder Celik, Yusuf Turkoz, Mehmet Hascalik, Yilmaz Cigremis, Bulent Mizrak, Saim Yologlu
• Journal: Gynecologic and obstetric investigation (Gynecol Obstet Invest) Vol. 57 Issue 4 Pg. 218-23 ( 2004) ISSN: 0378-7346 [Print] Switzerland
• PMID: 14970669 (Publication Type: Journal Article)
• Copyright: Copyright 2004 S. Karger AG, Basel

Chemical References

• Antioxidants
• Flavonoids
• Phenols
• Polyphenols
• Stilbenes
• Resveratrol

Topics

• Animals
• Antioxidants (therapeutic use)
• Female
• Flavonoids (therapeutic use)
• Ovarian Diseases (drug therapy)
• Ovary (blood supply, injuries, surgery)
• Phenols (therapeutic use)
• Phytotherapy
• Polyphenols
• Rats
• Rats, Wistar
• Reperfusion Injury (drug therapy)
• Resveratrol
• Stilbenes (therapeutic use)
• Torsion Abnormality
• Vitis
• Wine