[Analysis of cloning rearrangement of T-cell receptor gamma gene in primary cutaneous lymphoma].

Abstract

The aim of the study was to evaluate the usefulness of the T-cell receptor (TCR) gamma gene rearrangement analysis in the diagnosis of mycosis fungoides (MF) and Sezary syndrome (SS). The analysis of TCR gamma gene rearrangements was performed in patients with MF/SS in different stages and in subjects with inflammatory dermatoses as the control group, using the method of polymerase chain reaction with subsequent separation of products by temperature gradient gel electrophoresis. Dominant clones with TCR-gamma rearrangement were detected in 86.5% of MF/SS skin biopsies and in 67.5% of MF/SS peripheral blood cells whereas in control group in 12% and 15% respectively. Statistically significant differences were found in the occurrence of clonal T-cells in skin infiltrates between patients with MF/SS and control group. Statistical analysis of TCR-gamma rearrangement in peripheral blood cells did not revealed any differences only in patients with early stage (IA) of MF when compared with inflammatory dermatoses. Detection of T-cell receptor gamma gene rearrangement is a valid supplement to histopathological and immunohistochemical examination in cases suspected of MF/SS however the diagnosis should always be based on the analysis of examinations and clinical stage of patients.

• Authors: Mariola Pawlaczyk, Magdalena Kistowska, Elzbieta Poreba, Violetta Filas, Jan Breborowicz, Anna Goździcka-Józefiak
• Journal: Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego (Pol Merkur Lekarski) Vol. 15 Issue 89 Pg. 420-3 (Nov 2003) ISSN: 1426-9686 [Print] Poland
• Vernacular Title: Analiza klonalnych rearanzacji genu gamma receptora limfocytów T w pierwotnych chłoniakach skóry T-komórkowych.
• PMID: 14969134 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antibodies, Monoclonal

Topics

• Adult
• Aged
• Aged, 80 and over
• Antibodies, Monoclonal
• Biopsy
• Female
• Gene Rearrangement, T-Lymphocyte (genetics)
• Genes, T-Cell Receptor gamma (genetics)
• Humans
• Male
• Middle Aged
• Polymerase Chain Reaction
• Sezary Syndrome (genetics, pathology)
• Skin Neoplasms (genetics, pathology)