Abstract |
Neurones in the median preoptic nucleus ( MnPN) and the ventrolateral preoptic area (vlPOA) express immunoreactivity for c-Fos protein following sustained sleep, and display elevated discharge rates during both non-REM and REM sleep compared to waking. We evaluated the hypothesis that MnPN and vlPOA sleep-active neurones are GABAergic by combining staining for c-Fos protein with staining for glutamic acid decarboxylase (GAD). In a group of six rats exhibiting spontaneous total sleep times averaging 82.2 +/- 5.1% of the 2 h immediately prior to death, >75% of MnPN neurones that were Fos-immunoreactive (IR) were also GAD-IR. Similar results were obtained in the vlPOA. In a group of 11 rats exhibiting spontaneous sleep times ranging from 20 to 92%, the number of Fos + GAD-IR neurones in MnPN and vlPOA was positively correlated with total sleep time. Compared to control animals, Fos + GAD-IR cell counts in the MnPN were significantly elevated in rats that were sleep deprived for 24 h and permitted 2 h of recovery sleep. These findings demonstrate that a majority of MnPN and vlPOA neurones that express Fos-IR during sustained spontaneous sleep are GABAergic. They also demonstrate that sleep deprivation is associated with increased activation of GABAergic neurones in the MnPN and vlPOA.
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Authors | Hui Gong, Dennis McGinty, Ruben Guzman-Marin, Keng-Tee Chew, Darya Stewart, Ronald Szymusiak |
Journal | The Journal of physiology
(J Physiol)
Vol. 556
Issue Pt 3
Pg. 935-46
(May 01 2004)
ISSN: 0022-3751 [Print] England |
PMID | 14966298
(Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Proto-Oncogene Proteins c-fos
- gamma-Aminobutyric Acid
- Glutamate Decarboxylase
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Topics |
- Animals
- Brain
(cytology)
- Brain Chemistry
- Cell Count
- Electroencephalography
- Electromyography
- Glutamate Decarboxylase
(analysis)
- Immunohistochemistry
- Male
- Neurons
(cytology, metabolism, physiology)
- Preoptic Area
(chemistry, cytology, physiology)
- Proto-Oncogene Proteins c-fos
(analysis, metabolism, physiology)
- Rats
- Rats, Sprague-Dawley
- Sleep
(physiology)
- Sleep Deprivation
(metabolism, physiopathology)
- Sleep, REM
(physiology)
- Time Factors
- gamma-Aminobutyric Acid
(physiology)
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