[Effect of inhibiting protein kinase C on calcium sensitivity of contractile apparatus of vascular smooth muscle during vasospasms of different origins].

Abstract

The aim of the study was to investigate the role of protein kinase C (PKC) in changes in myofilament Ca(2+)-sensitivity of vascular smooth muscle cells (SMC) in rats at different vasospastic states: hypoxic pulmonary vasoconstriction, genetically determined hypertension, and hypertension resulted from ionizing radiation. All vasospastic states demonstrated rightward shifts in pCa-tension curves suggesting that myofilament Ca(2+)-sensitivity had increased. In chemically (beta-escin) skinned pulmonary artery, hypoxia-induced increase in myofilament Ca(2+)-sensitivity was completely abolished by PKC inhibitor chelerythrine. The similar results were demonstrated in skinned aorta SMC of spontaneously hypertensive rats where an increase in myofilament Ca(2+)-sensitivity was also abolished by PKC inhibitors chelerythrine and staurosporine. The chelerythrine partially inhibited myofilament Ca(2+)-sensitivity that had increased following gamma-radiation. The data suggest the key role of PKC activity in modulation of myofilament Ca(2+)-sensitivity in SMC. We conclude that PKC-mediated increase in myofilament Ca(2+)-sensitivity is one of the main mechanisms which contribute to the vasospasm of different genesis.

• Authors: O O Pavlova, V F Sahach, A I Solovĭov
• Journal: Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) (Fiziol Zh (1994)) Vol. 49 Issue 6 Pg. 31-7 ( 2003) ISSN: 2522-9028 [Print] Ukraine
• Vernacular Title: Vplyv blokady proteïnkinazy C na zminy kal'tsiievoï chutlyvosti skorotlyvoho aparatu sudynnykh gladen'kykh m'iaziv pry vazospastychnykh stanakh riznogo henezu.
• PMID: 14965035 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Alkaloids
• Benzophenanthridines
• Enzyme Inhibitors
• Phenanthridines
• chelerythrine
• Protein Kinase C
• Staurosporine
• Calcium

Topics

• Actin Cytoskeleton (drug effects, metabolism, radiation effects)
• Alkaloids
• Animals
• Benzophenanthridines
• Calcium (pharmacology)
• Cell Hypoxia
• Endothelium, Vascular (cytology)
• Enzyme Inhibitors (pharmacology)
• Gene Expression
• Hypertension (genetics, physiopathology)
• Male
• Muscle Contraction (drug effects, radiation effects)
• Muscle, Smooth, Vascular (enzymology, metabolism)
• Phenanthridines (pharmacology)
• Protein Kinase C (antagonists & inhibitors, drug effects, metabolism)
• Pulmonary Artery (cytology)
• Radiation, Ionizing
• Rats
• Rats, Inbred SHR
• Rats, Inbred WKY
• Staurosporine (pharmacology)