Abstract | BACKGROUND: METHODS AND RESULTS: Tissue segments were mounted in Ussing chambers under open circuit conditions. The basal potential difference in tissues from nine patients with cystic fibrosis was -3.6 mV (SE 0.3 mV), not different from tissues from 12 patients without cystic fibrosis of -3.6 mV (0.5 mV). Amiloride (10 microM) caused a significantly greater fall in potential difference in bronchi from patients with cystic fibrosis (83.5% (SE 2.9%)) than in those from controls (55.1% (7.1%)). Isoprenaline (100 microM) produced no significant change in bioelectric properties in non- cystic fibrosis tissues, but induced a 26.2% (6.3%) increase in potential difference in cystic fibrosis airways. The latter response was reduced by amiloride pretreatment. Mucosal chloride substituted Krebs-Henseleit solution caused no change in bioelectric properties in cystic fibrosis airways. Sodium substituted Krebs solution produced a substantial fall in potential difference similar in magnitude to that seen after amiloride. Isotropic flux measurements showed no significant differences between non- cystic fibrosis and cystic fibrosis airways. No net movement of Na+ or Cl- was detected under open circuit conditions in either group. CONCLUSIONS:
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Authors | E W Alton, D F Rogers, R Logan-Sinclair, M Yacoub, P J Barnes, D M Geddes |
Journal | Thorax
(Thorax)
Vol. 47
Issue 12
Pg. 1010-4
(Dec 1992)
ISSN: 0040-6376 [Print] England |
PMID | 1494762
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Amiloride
(pharmacology)
- Bronchi
(physiopathology)
- Cystic Fibrosis
(physiopathology)
- Heart-Lung Transplantation
- Humans
- Ion Transport
(physiology)
- Isoproterenol
(pharmacology)
- Membrane Potentials
(drug effects, physiology)
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