[Cytochrome P450 monooxygenase contents and activities in human liver microsomes prepared from Chinese adults].

Abstract

Seven human liver samples were collected in this study. Five of them were obtained from donors died from non-disease accident, one was taken from part of the normal tissue of a liver specimen obtained by partial hepatectomy in a case of hepatic cavernous hemangioma and the last one from postmortem died by road accident. All the liver pieces were stored at -75 degrees C immediately after removed from the body. Liver microsomes was prepared by differential ultracentrifugation. The contents of cytochrome P450 and cytochrome b5 in Chinese adult liver microsomes were 0.36 +/- 0.08 and 0.23 +/- 0.05 nmol.mg-1 protein (n = 7). The activities of aminopyrine and ethylmorphine N-demethylase, 7-ethoxycoumarin-O-deethylase, nifedipine oxidase and (-)-praziquantel hydroxylase were 1.07 +/- 0.23, 1.82 +/- 0.31, 0.30 +/- 0.10, 0.43 +/- 0.18 and 0.69 +/- 0.43 nmol.mg-1.min-1 (n = 7), respectively.

• Authors: K He, Y Z Quan, Z G Tu
• Journal: Yao xue xue bao = Acta pharmaceutica Sinica (Yao Xue Xue Bao) Vol. 27 Issue 8 Pg. 577-81 ( 1992) ISSN: 0513-4870 [Print] China
• PMID: 1481673 (Publication Type: Journal Article)

Chemical References

• Cytochromes b5
• Cytochrome P-450 Enzyme System
• Oxidoreductases
• CYP3A protein, human
• Cytochrome P-450 CYP3A

Topics

• Adult
• Asian People
• China
• Cytochrome P-450 CYP3A
• Cytochrome P-450 Enzyme System (metabolism)
• Cytochromes b5 (metabolism)
• Humans
• Male
• Microsomes, Liver (enzymology)
• Oxidoreductases (metabolism)