Abstract |
It is poorly understood why vaccines could not be developed for the control and prevention of African swine fever (ASF) virus infection. The aim of our study was to identify genes non-essential for ASF virus replication because there were indications that certain viral gene products, which apparently are non-essential for viral replication, conferred protection from death due to ASF. A cosmid library representing the genome of ASF virus strain France 64 was established and characterized. Then, in order to inactivate viral genes by insertion, the beta-galactosidase (beta-gal) gene was introduced either randomly or at specific locations of selected cloned DNA fragments. These constructions were transfected into cells which had been previously infected with a cell-culture-adapted viral strain in order to allow the generation of recombinant progeny virus. Viable recombinant progeny was identified by at least one of the following means: (1) expression of beta-gal; (2) detection of beta-gal specific DNA by plaque hybridization, and (3) absence of a functional product of the inactivated gene. Presently, we are characterizing a recombinant virus with an insertionally inactivated thymidine kinase gene.
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Authors | C Mettraux, M Ackermann, J D Tratschin, U Kihm |
Journal | Veterinary microbiology
(Vet Microbiol)
Vol. 33
Issue 1-4
Pg. 101-15
(Nov 1992)
ISSN: 0378-1135 [Print] Netherlands |
PMID | 1481351
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Viral
- Thymidine Kinase
- beta-Galactosidase
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Topics |
- African Swine Fever Virus
(genetics)
- Animals
- Cell Line
- Cloning, Molecular
- Cosmids
- DNA, Viral
(analysis)
- Gene Expression Regulation, Viral
- Gene Library
- Genes, Viral
- Mutagenesis, Insertional
- Nucleic Acid Hybridization
- Plasmids
- Restriction Mapping
- Thymidine Kinase
(genetics)
- Transfection
- Virus Replication
(genetics)
- beta-Galactosidase
(genetics)
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