Studies on thermophile products. V. Immunosuppressive profile in vitro of Bacillus stearothermophilus component, Fr.5-B.

Abstract

The immunosuppressive profile of Bacillus stearothermophilus UK563 component, Fr.5-B, is presented in in vitro studies. Fr.5-B (0.1-1000 ng/ml), provided it was added at the initiation of mixed leukocyte reaction (MLR), inhibited dose-dependently the incorporation of tritiated thymidine ([3H]TdR) into mouse spleen cells and human peripheral blood lymphocytes. Even the addition of Fr.5-B 48 h after the onset of culture suppressed mouse MLR, unlike cyclosporin A (CYA). Fr.5-B significantly inhibited cytotoxic T lymphocyte generation determined by [3H]TdR-release micro-cytotoxicity assay by using mouse mastocytoma P815 as targets. Moreover, this component decreased dose-dependently the expression of class II major histocompatibility molecules (Ia) on mouse peritoneal macrophages induced by concanavalin A supernatant. The present results revealed the unique immunosuppressive property of Fr.5-B which was different from that of CYA.

• Authors: Y Kohama, K Tanaka, N Murayama, M Itoh, T Suda, S Takae, T Mimura
• Journal: Chemical & pharmaceutical bulletin (Chem Pharm Bull (Tokyo)) Vol. 40 Issue 11 Pg. 3017-20 (Nov 1992) ISSN: 0009-2363 [Print] Japan
• PMID: 1477916 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Fr.5-B, Bacillus stearothermophilus UK563 component
• Histocompatibility Antigens Class II
• Immunosuppressive Agents
• Organic Chemicals
• Cyclosporine

Topics

• Animals
• Cells, Cultured
• Cyclosporine (pharmacology)
• Cytotoxicity, Immunologic (drug effects)
• Female
• Geobacillus stearothermophilus (chemistry)
• Histocompatibility Antigens Class II (analysis)
• Humans
• Immune Tolerance
• Immunosuppressive Agents (immunology, pharmacology)
• Lymphocyte Culture Test, Mixed
• Macrophages (drug effects, immunology)
• Mice
• Organic Chemicals
• T-Lymphocytes, Cytotoxic (drug effects, immunology)
• Tumor Cells, Cultured