Abstract | HYPOTHESIS: Studies indicate that a depressed wound immune function contributes to an increased rate of wound complications and impaired wound healing following trauma- hemorrhage (T-H). Androgen, ie, 5 alpha-dihydrotestosterone, is responsible for producing the depressed systemic cell-mediated immune responses following T-H in males. The aim of the present study was to determine whether depletion of 5 alpha-dihydrotestosterone in males before T-H has any salutary effects on wound immune cell function and wound healing in male mice following T-H. DESIGN: RESULTS: Precastration prevented the significantly suppressed capacity of wound immune cells to release IL-1 beta and IL-6. In addition, precastration normalized the elevated IL-6 expression at the wound site in the T-H mice. Moreover, wound-breaking strength was improved in castrated mice 10 days after T-H. CONCLUSIONS: Male sex steroids appear to be responsible for wound immune cell dysfunction following trauma and severe blood loss. Because decreasing androgen levels resulted in improved wound healing, our results suggest that the use of androgen receptor-blocking agents, eg, flutamide, following T-H might represent a novel adjunct for decreasing the rate of wound complications under those conditions.
|
Authors | Stefan M Nitsch, Florian Wittmann, Peter Angele, Matthias W Wichmann, Rudolf Hatz, Thomas Hernandez-Richter, Irshad H Chaudry, Karl W Jauch, Martin K Angele |
Journal | Archives of surgery (Chicago, Ill. : 1960)
(Arch Surg)
Vol. 139
Issue 2
Pg. 157-63
(Feb 2004)
ISSN: 0004-0010 [Print] United States |
PMID | 14769573
(Publication Type: Comparative Study, Journal Article)
|
Chemical References |
- Cytokines
- Dihydrotestosterone
|
Topics |
- Analysis of Variance
- Animals
- Castration
- Cytokines
(analysis)
- Dihydrotestosterone
(blood, metabolism)
- Disease Models, Animal
- Immunity, Cellular
(physiology)
- Immunohistochemistry
- Male
- Mice
- Mice, Inbred C3H
- Probability
- Random Allocation
- Reference Values
- Sensitivity and Specificity
- Shock, Hemorrhagic
(immunology, therapy)
- Wound Healing
(immunology, physiology)
- Wounds and Injuries
(immunology, pathology, therapy)
|