Abstract | AIM: METHODS:
Brain ischemia was induced by four-vessel occlusion procedure in rats. Immunoprecipitation and immunoblotting were performed to study the interactions and phosphorylation of proteins. The association-dissociation of PSD-95 and CaMKIIalpha to and from N-methyl-D-aspartate ( NMDA) receptor induced by ischemia and reperfusion and the effects of 1-[N,O-bis-(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenyl- piperazine ( KN-62, a selective inhibitor of CaMKII) on these protein interactions were investigated. Coimmunoprecipitation and immunoblotting were performed for the studies of interactions among proteins. RESULTS: The alternations of the binding level of PSD-95 and CaMKIIalpha to NR2B during ischemia and reperfusion demonstrated the negative correlation to each other. Pre-administration of KN62 through both cerebral ventricles inhibited the 10 min ischemia-induced increase of the binding of PSD-95 to NR2B and, on the contrary, promoted the binding of CaMKIIalpha to NR2B. CONCLUSION: PSD-95 competes with CaMKII to bind to NR2B during ischemia and reperfusion in rat hippocampus.
|
Authors | Fan-jie Meng, Jun Guo, Bo Song, Xue-bo Yan, Guang-yi Zhang |
Journal | Acta pharmacologica Sinica
(Acta Pharmacol Sin)
Vol. 25
Issue 2
Pg. 176-80
(Feb 2004)
ISSN: 1671-4083 [Print] United States |
PMID | 14769205
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Disks Large Homolog 4 Protein
- Dlg4 protein, rat
- Intracellular Signaling Peptides and Proteins
- Membrane Proteins
- Nerve Tissue Proteins
- Receptors, N-Methyl-D-Aspartate
- postsynaptic density proteins
- KN 62
- 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
- Calcium-Calmodulin-Dependent Protein Kinase Type 2
- Calcium-Calmodulin-Dependent Protein Kinases
- Camk2a protein, rat
|
Topics |
- 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
(analogs & derivatives, pharmacology)
- Animals
- Binding, Competitive
- Brain Ischemia
(complications)
- Calcium-Calmodulin-Dependent Protein Kinase Type 2
- Calcium-Calmodulin-Dependent Protein Kinases
(antagonists & inhibitors, metabolism)
- Disks Large Homolog 4 Protein
- Hippocampus
(metabolism)
- Intracellular Signaling Peptides and Proteins
- Male
- Membrane Proteins
- Nerve Tissue Proteins
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Receptors, N-Methyl-D-Aspartate
(metabolism)
- Reperfusion Injury
(etiology, metabolism)
|