Abstract | BACKGROUND: METHODS: Plasma levels of CML and CEL were measured in 60 type 1 diabetic patients categorized as having normal glomerular filtration rate (GFR) (>80 ml/min, n = 31) or decreased GFR (<80 ml/min, n = 29) as estimated by the Cockcroft-Gault formula. To assess the relationship of these AGEs to endothelial function and inflammation, markers of endothelial function von Willebrand factor (vWf), soluble vascular cellular adhesion molecule-1 (sVCAM-1), soluble E-selectin (sE- selectin), soluble thrombomodulin (sTM), tissue type-specific plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1), and C-reactive protein (CRP), a marker of inflammatory activity, were determined by enzyme-linked immunosorbent assays. RESULTS: Plasma levels of CML and CEL were increased in diabetic patients with decreased GFR as compared with patients with normal GFR [CML 4.9 (2-12.6) vs 2.9 (1.7-4.4) micromol/l, P<0.000; and CEL 1.7 (0.9-3.3) vs 1.2 (1.7-4.4) micromol/l, P = 0.004, respectively). Independently of the GFR, the plasma levels of CML and CEL were significantly associated with sVCAM-1, vWf and sTM. CONCLUSIONS: Plasma levels of CML and CEL rise with deterioration of GFR. Furthermore, CML and CEL levels are associated with markers of endothelial activation independently of renal function. This suggests an involvement of these AGEs in the acceleration of cardiovascular complications in patients with renal impairment.
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Authors | Mariska L M Lieuw-A-Fa, Victor W M van Hinsbergh, Tom Teerlink, Rob Barto, Jos Twisk, Coen D A Stehouwer, Casper G Schalkwijk |
Journal | Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
(Nephrol Dial Transplant)
Vol. 19
Issue 3
Pg. 631-6
(Mar 2004)
ISSN: 0931-0509 [Print] England |
PMID | 14767019
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Blood Coagulation Factors
- E-Selectin
- Thrombomodulin
- Vascular Cell Adhesion Molecule-1
- N(6)-(1-carboxyethyl)lysine
- N(6)-carboxymethyllysine
- C-Reactive Protein
- Lysine
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Topics |
- Adult
- Aged
- Blood Coagulation Factors
(metabolism)
- C-Reactive Protein
(metabolism)
- Case-Control Studies
- Diabetes Mellitus, Type 1
(blood)
- E-Selectin
(blood)
- Endothelium, Vascular
(physiopathology)
- Female
- Glomerular Filtration Rate
(physiology)
- Humans
- Kidney
(physiopathology)
- Lysine
(analogs & derivatives, blood)
- Male
- Middle Aged
- Thrombomodulin
(blood)
- Vascular Cell Adhesion Molecule-1
(blood)
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