Abstract | OBJECTIVE: METHODS: We performed heterologous, left lung transplantation (+4 degrees C ischemia; 24 hours, Euro-Collins solution) in 6 foxhounds (untreated) and in 6 animals that received calf lung surfactant extract ( Alveofact) prior to explantation (only donor lung; 50 mg/kg body weight) and immediately after onset of reperfusion (both lungs, 200 mg/kg body weight). Separate but synchronized ventilation of each lung was performed, in a volume-controlled, pressure-limited mode, with animals in prone position. Bronchoalveolar lavage fluids were collected in pretransplantation lungs (control), after 24 hours of ischemia prior to transplantation (0 hours) and 6 and 12 hours after reperfusion in both the grafts and the recipient native lungs. RESULTS: Ischemic storage per se did not provoke any changes of the surfactant system; however, severe alterations occurred within 6 hours of reperfusion, resulting in a severe loss of surface activity, including a decrease in the percentage of the large surfactant aggregate fraction, reduction of the surfactant apoproteins SP-B and SP-C, the dipalmitoyl molecular species of phosphatidylcholine and phosphatidylglycerol within the large surfactant aggregate fraction. These abnormalities were restricted to the graft, with virtually normal surfactant function and composition being found in the recipient native lung. Surfactant administration fully normalized the biochemical and largely improved the biophysical surfactant properties, alongside maintenance of lung gas exchange properties. CONCLUSIONS:
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Authors | Andreas Günther, Markus Balser, Reinhold Schmidt, Philipp Markart, Alexander Olk, Jochen Börgermann, Fred H Splittgerber, Werner Seeger, Ivar Friedrich |
Journal | The Journal of thoracic and cardiovascular surgery
(J Thorac Cardiovasc Surg)
Vol. 127
Issue 2
Pg. 344-54
(Feb 2004)
ISSN: 0022-5223 [Print] United States |
PMID | 14762341
(Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apoproteins
- Biomarkers
- Lipids
- Phospholipids
- Proteins
- Pulmonary Surfactant-Associated Proteins
- Pulmonary Surfactants
- SF-RI 1, bovine surfactant preparation
- pulmonary surfactant apoprotein
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Topics |
- Animals
- Apoproteins
(administration & dosage)
- Biomarkers
(analysis)
- Bronchoalveolar Lavage Fluid
(chemistry)
- Bronchoscopy
- Disease Models, Animal
- Dogs
- Dose-Response Relationship, Drug
- Female
- Lipids
(administration & dosage)
- Lung Transplantation
- Male
- Models, Biological
- Phospholipids
(administration & dosage, metabolism)
- Postoperative Complications
(etiology, metabolism, physiopathology)
- Proteins
(metabolism)
- Pulmonary Alveoli
(abnormalities, drug effects, physiopathology)
- Pulmonary Surfactant-Associated Proteins
(administration & dosage)
- Pulmonary Surfactants
(administration & dosage)
- Reperfusion Injury
(etiology, metabolism, physiopathology)
- Severity of Illness Index
- Statistics as Topic
- Surface Tension
(drug effects)
- Time Factors
- Transplantation, Heterologous
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