| Abstract | OBJECTIVE: Mycobacterium vaccae injected subcutaneously was previously shown to prevent and treat ligature-induced periodontal disease (PD) in Wistar rats (Breivik & Rook 2000, 2002). Since mycobacteria are readily taken up via Peyer's patches in the intestine, we have now tested the ability of oral SRP299 (killed M. vaccae) to prevent ligature-enhanced PD in Wistar rats, and to modulate the accompanying cytokine and corticosterone responses. MATERIAL AND METHODS: A single oral dose of SRP299 (1 mg) was given 14 days before the application of ligatures. PD was assessed when the ligatures had been in place for 56 days. RESULTS: Oral SRP299 reduced bone loss (p = 0.036, X-ray; p = 0.061, histometry) and fibre loss, both on the ligatured (p = 0.0047) and control (p = 0.005) sides, and also reduced the level of TNF-alpha (p = 0.0137) and corticosterone (p = 0.048) induced by intraperitoneal endotoxin lipopolysaccharide (LPS). CONCLUSIONS: SRP299 administered by the oral route diminishes ligature-induced bone and fibre loss in this model. This effect may be attributable to the known ability of SRP299 to evoke regulatory T cells, although the mechanism could not be investigated in this study. Treated rats also had less excitable hypothalamo-pituitary-adrenal (HPA) axes. HPA axis overactivity is a known risk factor in human PD. Trials of SRP299 in human PD are now justified. |
| Authors | Torbjørn Breivik, Graham A W Rook
(Affiliation: Department of Periodontology, Faculty of Dentistry, University of Oslo, Blindern, Oslo, Norway. tbreivik at odont.uio.no)
|
| Journal | Journal of clinical periodontology
(J Clin Periodontol)
Vol. 30
Issue 11
Pg. 931-6
(Nov 2003)
ISSN: 0303-6979 Denmark |
| PMID | 14761113
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Bacterial Vaccines
- SRP 299
- Tumor Necrosis Factor-alpha
- Vaccines, Inactivated
- Interleukin-10
- Corticosterone
|
| Topics |
- Administration, Oral
- Alveolar Bone Loss
(immunology, prevention & control)
- Animals
- Bacterial Vaccines
(administration & dosage, immunology)
- Corticosterone
(blood)
- Interleukin-10
(blood)
- Male
- Maxilla
- Molar
- Mycobacterium
(immunology)
- Mycobacterium Infections
(prevention & control)
- Periodontal Diseases
(immunology, prevention & control)
- Periodontal Ligament
(immunology, pathology)
- Rats
- Rats, Wistar
- Serum
(chemistry)
- Single-Blind Method
- Statistics, Nonparametric
- Tumor Necrosis Factor-alpha
(analysis)
- Vaccines, Inactivated
|
