Polyozellin isolated from Polyozellus multiplex induces phase 2 enzymes in mouse hepatoma cells and differentiation in human myeloid leukaemic cell lines.

Abstract

Induction of cellular phase 2 detoxifying enzymes is associated with cancer preventive potential. Quinone reductase (QR) has been used as a prototype for anticarcinogenic phase 2 enzymes because of its widespread distribution in mammalian systems, large amplitude of inducer response, and ease of measurement in murine hepatoma cells. Methanol extract of Polyozellus multiplex, which shows a strong QR induction potential, was subjected to bioassay-guided fractionation, and polyozellin (PM1) appeared to be a major active component. In in vitro cultured cells (hepa1c1c7 and BPRc1 cells), polyozellin enhanced QR, glutathione S-transferase (GST) activities, and glutathione (GSH) content in a dose-dependent manner. The compound also significantly promoted differentiation of HL-60 human promyelocytic emia cells. In conclusion, polyozellin deserves further in vivo study to evaluate its potential as a cancer preventive agent.

• Authors: Jeong Hyun Kim, Jeong Soon Lee, Kyung-Sik Song, Chong-Suk Kwon, Young Kyoon Kim, Jong-Sang Kim
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 52 Issue 3 Pg. 451-5 (Feb 11 2004) ISSN: 0021-8561 [Print] United States
• PMID: 14759131 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anticarcinogenic Agents
• Furans
• polyozellin
• Glutathione Transferase
• Glutathione
• Methanol

Topics

• Agaricales (chemistry)
• Animals
• Anticarcinogenic Agents
• Cell Differentiation (drug effects)
• Dose-Response Relationship, Drug
• Enzyme Induction (drug effects)
• Furans (isolation & purification, pharmacology)
• Glutathione (analysis)
• Glutathione Transferase (biosynthesis)
• Humans
• Leukemia, Myeloid (pathology)
• Liver Neoplasms, Experimental (enzymology)
• Methanol
• Mice
• Tumor Cells, Cultured