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Inhibitory effect of tellimagrandin I on chemically induced differentiation of human leukemia K562 cells.

Abstract
Tellimagrandin I is a hydrolysable tannin compound widely present in plants. In this study, the effect of tellimagrandin I on chemically induced erythroid and megakaryocytic differentiation was investigated using K562 cells as differentiation model. It was found that tellimagrandin I not only inhibited the hemoglobin synthesis in butyric acid (BA)- and hemin-induced K562 cells with IC50 of 3 and 40microM, respectively, but also inhibited other erythroid differentiation marker including acetylcholinesterase (AChE) and glycophorin A (GPA) in BA-induced K562 cells. Tellimagrandin I also inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced expression of CD61 protein, a megakaryocytic marker. RT-PCR analysis showed that tellimagrandin I decreased the expression of erythroid genes (gamma-globin and porphobilinogen deaminase (PBGD)) and related transcription factors (GATA-1 and NF-E2) in BA-induced K562 cells, whereas tellimagrandin I induced the overexpresison of GATA-2 transcription factor that played negative regulation on erythroid differentiation. These results indicated that tellimagrandin I had inhibitory effects on erythroid and megakaryocytic differentiation, which suggested that tannins like tellimagrandin I might influence the anti-tumor efficiency of some drugs and the hematopoiesis processes.
AuthorsZongchun Yi, Zhao Wang, Haixia Li, Mingjie Liu
JournalToxicology letters (Toxicol Lett) Vol. 147 Issue 2 Pg. 109-19 (Mar 01 2004) ISSN: 0378-4274 [Print] Netherlands
PMID14757314 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Benzidines
  • Cholinesterase Inhibitors
  • DNA Primers
  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • GATA1 protein, human
  • GATA2 Transcription Factor
  • GATA2 protein, human
  • Glucosides
  • Glycophorins
  • Hemoglobins
  • NF-E2 Transcription Factor
  • NF-E2 Transcription Factor, p45 Subunit
  • NFE2 protein, human
  • RNA, Messenger
  • Transcription Factors
  • benzidine
  • Gallic Acid
  • tellimagrandin I
  • Hydroxymethylbilane Synthase
  • Acetylcholinesterase
  • Tetradecanoylphorbol Acetate
Topics
  • Acetylcholinesterase (metabolism)
  • Benzidines (chemistry)
  • Cell Differentiation (drug effects)
  • Cholinesterase Inhibitors (pharmacology)
  • DNA Primers
  • DNA-Binding Proteins (physiology)
  • Erythroid Precursor Cells (drug effects)
  • Erythroid-Specific DNA-Binding Factors
  • Flow Cytometry
  • GATA1 Transcription Factor
  • GATA2 Transcription Factor
  • Gallic Acid (analogs & derivatives, pharmacology)
  • Glucosides (pharmacology)
  • Glycophorins (metabolism)
  • Hematopoiesis (drug effects)
  • Hemoglobins (biosynthesis)
  • Humans
  • Hydroxymethylbilane Synthase (metabolism)
  • K562 Cells
  • Megakaryocytes (drug effects)
  • NF-E2 Transcription Factor
  • NF-E2 Transcription Factor, p45 Subunit
  • RNA, Messenger (biosynthesis)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tetradecanoylphorbol Acetate (pharmacology)
  • Transcription Factors (physiology)

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