Phylogenetic conservation of a snake venom metalloproteinase epitope recognized by a monoclonal antibody that neutralizes hemorrhagic activity.

Abstract

Snake venom metalloproteinases (SVMPs) are present in large quantities in venoms of viper snakes and also in some elapids. Jararhagin is a representative of a P-III multidomain hemorrhagic SVMP present in Bothrops jararaca venom. It is comprised of a catalytic, a disintegrin-like and a cysteine-rich domain. Seven anti-jararhagin monoclonal antibodies (MAJar 1-7) were produced, of which six reacted with the disintegrin domain. MAJar 3 recognized an epitope present at the C-terminal part of the disintegrin-like domain, and neutralized jararhagin-induced hemorrhage. In this study, we evaluated the reactivity of these monoclonal antibodies with venoms from 27 species of snakes belonging to different families. MAJar 3 recognized most of the hemorrhagic venoms. By ELISA, MAJar 3 reacted strongly with venoms from Viperidae family and weakly with Colubridae and Elapidae venoms. This recognition pattern was due to bands between 50 and 80 kDa, corresponding to P-III SVMPs. This antibody preferentially neutralized the hemorrhage induced by venoms of Bothrops snakes. This fact suggests that the epitope recognized by MAJar 3 is present in other metalloproteinases throughout snake phylogeny. However, slight structural differences in the epitope may result in insufficient affinity for neutralization of biological activities.

• Authors: Isabelle Tanjoni, Diego Butera, Patrick J Spencer, Harumi A Takehara, Irene Fernandes, Ana Maria Moura-da-Silva
• Journal: Toxicon : official journal of the International Society on Toxinology (Toxicon) Vol. 42 Issue 7 Pg. 809-16 (Dec 2003) ISSN: 0041-0101 [Print] England
• PMID: 14757213 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Crotalid Venoms
• Epitopes
• Metalloproteases
• Metalloendopeptidases
• jararhagin

Topics

• Amino Acid Sequence
• Animals
• Antibodies, Monoclonal (immunology, pharmacology)
• Blotting, Western
• Bothrops (classification, genetics, immunology)
• Colubridae (genetics, immunology)
• Crotalid Venoms (chemistry, enzymology, genetics, immunology, toxicity)
• Elapidae (genetics, immunology)
• Enzyme-Linked Immunosorbent Assay
• Epitopes (chemistry, genetics, immunology)
• Hemorrhage (chemically induced, immunology, prevention & control)
• Metalloendopeptidases (chemistry, genetics, immunology)
• Metalloproteases (chemistry, genetics, immunology)
• Mice
• Molecular Sequence Data
• Phylogeny
• Sequence Alignment
• Viperidae (genetics, immunology)