Abstract | OBJECTIVE: METHODS: The rat model of acute intrauterine ischemic- hypoxia was established by ligating the unilateral uterine horn vessels of Wistar rats at the 21st gestational day. While the rat pups from the other side of the uterus, of which the uterine horn vessel was not ligated, were the sham-operation group. Rat pups were delivered by cesarean section at the 20, 30 and 40 min following the ischemic- hypoxia insult. The rat pups delivered by cesarean section from the gestation of 21 days were the normal control group. There were 42 rat pups and 6 pups in each group in this study. The distribution of SP-B protein in the neonatal rat lungs of different period of ischemia was examined by using SABC method. The average gray value of SP-B staining in type II alveolar epithelial cells were measured by Universal Imaging Porporation with Meta Morph software. The reverse transcription polymerase chain reaction (RT-PCR) was performed to quantitate the expression of SP-A and SP-B mRNA. RESULTS: Following the intrauterine acute ischemic- hypoxia, the numbers of type II alveolar epithelial cells with the positive SP-B staining were markedly declined. The average gray values at the 20, 30 and 40 min after the ischemia were 78.89 +/- 1.08, 79.69 +/- 0.13 and 80.00 +/- 0.63, respectively, which increased significantly compared with the normal control group (76.13 +/- 0.43, P < 0.01). The expression of SP-A and SP-B mRNA was weak following the ischemic- hypoxia insult. The relative amounts of SP-A (1.16 +/- 0.06, 1.14 +/- 0.01 and 1.13 +/- 0.04, respectively) and SP-B (0.81 +/- 0.02, 0.78 +/- 0.02 and 0.79 +/- 0.04, respectively) at the 20, 30 and 40 min after the ischemia were reduced significantly compared with controls (1.27 +/- 0.09 and 0.89 +/- 0.06, respectively, P < 0.05 and < 0.01) and reduced gradually following the prolongation of the insult. There were no significant differences (P > 0.05) between the normal and sham operation control groups on the expressions of SP-B protein as well as the SP-A and SP-B mRNA. CONCLUSION:
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Authors | Xu-xu Cai, Yue Du, Hong Gao, Zhen-ze Cui, Yu-kun Han |
Journal | Zhonghua er ke za zhi = Chinese journal of pediatrics
(Zhonghua Er Ke Za Zhi)
Vol. 41
Issue 3
Pg. 208-11
(Mar 2003)
ISSN: 0578-1310 [Print] China |
PMID | 14756961
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Pulmonary Surfactant-Associated Protein A
- Pulmonary Surfactant-Associated Protein B
- RNA, Messenger
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Topics |
- Animals
- Animals, Newborn
- Female
- Gene Expression
- Hypoxia
(physiopathology)
- Ischemia
(physiopathology)
- Lung
(metabolism)
- Pregnancy
- Pulmonary Surfactant-Associated Protein A
(genetics)
- Pulmonary Surfactant-Associated Protein B
(genetics)
- RNA, Messenger
(genetics, metabolism)
- Rats
- Rats, Wistar
- Reverse Transcriptase Polymerase Chain Reaction
- Uterus
(blood supply)
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