[Cytokines and bone turnover markers].

Abstract

OBJECTIVE:

Bone turnover markers (osteocalcin, bone-ALP, beta-crosslaps--CTX) and cytokines (IL-1 alpha, IL-8 and IL-10) in hip joint fluid were analyzed in patients with aseptic loosening of prosthesis before revision surgery, in patients with rheumatoid arthritis and with idiopathic coxarthrosis for comparison.

METHODS:

Bone turnover markers were determined by electrochemiluminescence, colorimetric or ELISA method and cytokines by ELISA in joint fluid collected at the beginning of the surgery.

RESULTS:

Patients with loose implants had lower concentration of the resorption marker than cases with rheumatoid arthritis and coxarthrosis (7771 +/- 3322 vs 25986 +/- 16059 p < 0.01 and 23047 +/- 32556 pmol/L, p < 0.003) and over tenfold lower concentration of osteocalcin, the bone formation marker (p < 0.04 i p < 0.01). Concentration of IL-8 was elevated and similar in patients with loosening and rheumatoid arthritis while in cases with osteoarthrosis the mean value was twice lower. The anti-inflammatory IL-10 was highly elevated only in cases with prosthesis loosening. Additionally, a negative correlation was observed between CTX and IL-10 was and positive between IL-10 and time to revision surgery.

CONCLUSIONS:

We conclude that increased local production of inflammatory cytokines leading to uncoupling of bone turnover is a part of the loosening process.

• Authors: Grazyna Odrowaz-Sypniewska, Kinga Lis, Piotr J Biliński
• Journal: Przeglad epidemiologiczny (Przegl Epidemiol) Vol. 56 Suppl 4 Pg. 39-45 ( 2002) ISSN: 0033-2100 [Print] Poland
• Vernacular Title: Cytokiny i markery obrotu kostnego--badania własne.
• PMID: 14750259 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Biomarkers
• Cytokines
• Interleukin-1
• Interleukin-8
• Peptide Fragments
• glutamyl-lysyl-alanyl-histidyl-aspartyl-glycyl-glycyl-arginine
• Osteocalcin
• Interleukin-10
• Collagen
• Alkaline Phosphatase

Topics

• Aged
• Aged, 80 and over
• Alkaline Phosphatase (analysis)
• Arthritis, Rheumatoid (surgery)
• Arthroplasty, Replacement, Hip (adverse effects)
• Biomarkers (analysis)
• Bone Resorption (etiology, immunology)
• Collagen (analysis)
• Colorimetry
• Cytokines (analysis)
• Enzyme-Linked Immunosorbent Assay
• Female
• Hip Joint (chemistry)
• Humans
• Interleukin-1 (analysis)
• Interleukin-10 (analysis)
• Interleukin-8 (analysis)
• Male
• Middle Aged
• Osteoarthritis, Hip (surgery)
• Osteocalcin (analysis)
• Peptide Fragments (analysis)
• Prosthesis Failure
• Synovial Fluid (chemistry)