| Abstract | Adipose tissue represents an important source of angiotensinogen (AGT). We investigated the effect of obesity induced by a high-fat diet on the expression of mouse (mAGT) and human AGT (hAGT) genes in liver, kidney, and heart and different adipose depots in normal mice (C57BL/6J), and in transgenic mice expressing the hAGT gene under the control of its own promoter. Mice were fed a high-fat diet (45% kcal) or normal chow (10% kcal) for 10 and 20 wk. The expression of mAGT and hAGT mRNA was quantified using an RNAse protection assay. Mice on the high-fat diet exhibited increased weight, fat mass, and plasma leptin. Expression of mAGT or hAGT genes was not affected by high-fat diet in nonadipose tissues, brown adipose tissue, or subcutaneous white fat. In contrast, high-fat diet increased both mAGT and hAGT gene expression in visceral adipose depots (omental, reproductive, and perirenal fat). Thus obesity-induced by a high-fat diet is associated with a tissue-specific increased expression of both mouse and human AGT genes in intra-abdominal adipose tissue. Our findings also suggest that 1.2 kb of regulatory sequences present in the hAGT transgene are sufficient to transcriptionally respond to a high-fat diet in an adipose-specific and depot-specific manner. |
| Authors | Kamal Rahmouni, Allyn L Mark, William G Haynes, Curt D Sigmund
(Affiliation: Hypertension Genetics Specialized Center of Research, Cardiovascular Center, Department of Internal Medical, University of Iowa Carver College of Medicine, Iowa City 52242, USA.)
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| Journal | American journal of physiology. Endocrinology and metabolism
(Am J Physiol Endocrinol Metab)
Vol. 286
Issue 6
Pg. E891-5
(Jun 2004)
ISSN: 0193-1849 United States |
| PMID | 14749209
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
| Chemical References |
- Dietary Fats
- Leptin
- RNA, Messenger
- Angiotensinogen
|
| Topics |
- Adipose Tissue
(physiology)
- Angiotensinogen
(genetics)
- Animals
- Body Weight
- Dietary Fats
(pharmacology)
- Gene Expression
- Hypertension
(physiopathology)
- Leptin
(blood)
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Obesity
(physiopathology)
- RNA, Messenger
(analysis)
|
