Abstract | BACKGROUND: OBJECTIVES: To investigate the effects of DLC on melanin synthesis using cultured human melanoma cells. METHODS: RESULTS: At concentrations of 1.4-14 micromol L-1, DLC reduced the pigmentation of HM3KO melanoma cells but did not affect cell growth. The visual decrease in pigmentation produced by DLC was more dramatic than the decrease in total melanin content as measured by absorbance at 500 nm. DLC treatment decreased eumelanin synthesis and increased phaeomelanin synthesis in HM3KO melanoma cells. An in situ tyrosinase assay showed that DLC inhibited tyrosinase activity, as well as the level of tyrosinase protein. CONCLUSIONS: These results suggest that DLC has pigment-lightening effects on HM3KO melanoma cells, produced by reducing the level of eumelanin while increasing the level of phaeomelanin.
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Authors | J S Hwang, H Choi, H S Rho, H J Shin, D H Kim, J Lee, B G Lee, I Chang |
Journal | The British journal of dermatology
(Br J Dermatol)
Vol. 150
Issue 1
Pg. 39-46
(Jan 2004)
ISSN: 0007-0963 [Print] England |
PMID | 14746615
(Publication Type: Journal Article)
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Chemical References |
- Enzyme Inhibitors
- Melanins
- N,N'-dilinoleylcystamine
- Monophenol Monooxygenase
- Cystamine
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Topics |
- Blotting, Western
- Cystamine
(analogs & derivatives, pharmacology)
- Dose-Response Relationship, Drug
- Enzyme Inhibitors
(pharmacology)
- Humans
- Image Processing, Computer-Assisted
(methods)
- Melanins
(biosynthesis)
- Melanoma
(metabolism, pathology)
- Monophenol Monooxygenase
(antagonists & inhibitors, metabolism)
- Pigmentation
(drug effects)
- Skin Pigmentation
- Tumor Cells, Cultured
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