Chondroitin sulfate of appican, the proteoglycan form of amyloid precursor protein, produced by C6 glioma cells interacts with heparin-binding neuroregulatory factors.

Appican produced by rat C6 glioma cells, the chondroitin sulfate (CS) proteoglycan form of the amyloid precursor protein, contains an E disaccharide, -GlcUA-GalNAc(4,6-O-disulfate)-, in its CS chain. In this study, the appican CS chain from rat C6 glioma cells was shown to specifically bind several growth/differentiation factors including midkine (MK) and pleiotrophin (PTN). In contrast, the appican CS from SH-SY5Y neuroblastoma cells contained no E disaccharide and showed no binding to either MK or PTN. These findings indicate that the E motif is essential in the interaction of the appican CS chain with growth/differentiation factors, and suggest that glial appican may mediate the regulation of neuronal cell adhesion and migration and/or neurite outgrowth.
AuthorsYuko Umehara, Shuhei Yamada, Shuji Nishimura, Junichi Shioi, Nikolaos K Robakis, Kazuyuki Sugahara
JournalFEBS letters (FEBS Lett) Vol. 557 Issue 1-3 Pg. 233-8 (Jan 16 2004) ISSN: 0014-5793 [Print] Netherlands
PMID14741373 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Amyloid beta-Protein Precursor
  • Carrier Proteins
  • Cytokines
  • Glycosaminoglycans
  • Neoplasm Proteins
  • Nerve Tissue Proteins
  • Oligosaccharides
  • Proteoglycans
  • appican
  • pleiotrophin
  • midkine
  • Heparin
  • Amyloid beta-Protein Precursor
  • Carbohydrate Sequence
  • Carrier Proteins (metabolism)
  • Cytokines (metabolism)
  • Glioma
  • Glycosaminoglycans (chemistry, metabolism)
  • Heparin (metabolism)
  • Humans
  • Molecular Sequence Data
  • Neoplasm Proteins (metabolism)
  • Nerve Tissue Proteins (metabolism)
  • Neuroblastoma
  • Oligosaccharides (chemistry)
  • Proteoglycans (biosynthesis, chemistry, metabolism)
  • Tumor Cells, Cultured

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