[The study of cucurbitacin BE polylactic acid nanoparticles delivering cucurbitacin BE to metastasized cervical lymph nodes in mice with oral cancer].

Abstract

OBJECTIVE:

The aim of this study was to investigate the delivery of Cucurbitacin BE to cervical lymph nodes by peri-cancer submucosal injection of the average diameter 85 nm Cucurbitacin BE polylactic acid nanoparticles (CuBE-PLA-NP).

METHODS:

Cervical lymph node metastasis model of mice oral cancer was established and CuBE-PLA-NP and CuBE were injected in peri-cancer submucosal of the mice respectively, the concentration in blood, heart, liver, spleen, lung, kidney and cervical lymph node were measured by high performance liquid chromatography at fifteen time points, Targeted cervical lymph nodes were evaluated by targeting index, selectivity index, targeting efficiency, relative targeting efficiency and ratio of maximum concentration.

RESULTS:

(1) The CuBE concentration in the cervical lymph nodes after CuBE-PLA-NP injection was far higher than that after CuBE injection at every time point, the CuBE duration in the cervical lymph nodes in CuBE-PLA-NP group was far longer than that in CuBE group; (2) The five targeted values of the cervical lymph nodes in CuBE-PLA-NP group was far higher than that in CuBE group; (3) The CuBE concentration in blood, heart, liver, spleen, lung and kidney in CuBE-PLA-NP group was far lower than that in CuBE group.

CONCLUSION:

The CuBE-PLA-NP can specifically deliver CuBE to the cervical lymph nodes by peri-cancer submucosal injection, increase CuBE concentration and duration in metastasized cervical lymph nodes, and decrease drug concentration of other organs.

• Authors: Kai Yang, Yuming Wen, Changmei Wang
• Journal: Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology (Hua Xi Kou Qiang Yi Xue Za Zhi) Vol. 21 Issue 6 Pg. 477-80 (Dec 2003) ISSN: 1000-1182 [Print] China
• PMID: 14732987 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Delayed-Action Preparations
• Drug Carriers
• Polyesters
• Polymers
• Triterpenes
• Lactic Acid
• poly(lactide)
• Cucurbitacins

Topics

• Animals
• Antineoplastic Agents, Phytogenic (administration & dosage, pharmacokinetics)
• Carcinoma, Squamous Cell (drug therapy, surgery)
• Cucurbitacins
• Delayed-Action Preparations
• Drug Carriers
• Drug Delivery Systems
• Female
• Lactic Acid (administration & dosage, pharmacokinetics)
• Lymph Nodes (metabolism, pathology)
• Lymphatic Metastasis
• Male
• Mice
• Mouth Neoplasms (drug therapy, surgery)
• Nanotechnology
• Neck
• Particle Size
• Polyesters
• Polymers (administration & dosage, pharmacokinetics)
• Triterpenes (administration & dosage, pharmacokinetics)