Potential role for antiangiogenic proteins in the myocardial infarction repair process.

Although angiogenic proteins have been identified as positive modulators of myocardial revascularization following acute myocardial infarction, little if anything is known regarding the role that antiangiogenic proteins have in myocardial revascularization. We explored the temporospatial distribution of endothelial-monocyte activating polypeptide (EMAP) II to determine whether antiangiogenic proteins have a role in the repair of myocardial tissue following infarction.
A rat model of myocardial infarction was utilized to examine EMAP II distribution (in situ hybridization) and protein expression (Western analysis) over a 6-week period.
At baseline, EMAP II protein and mRNA are minimally expressed with transcription products localizing predominately to the perivascular stroma region in the normal rat myocardium. Six hours following myocardial infarction, EMAP II changes its distribution from the perivascular stroma to an invading inflammatory cell population. This is associated with a 2-fold (P < 0.0009) increase in EMAP II protein and its transcription primarily localized to the infarct region. EMAP II protein expression remains elevated throughout the weeks following the infarction with transcription limited to the infarct region and a notable decrease in EMAP II transcription products noted in the viable vasculature surrounding the infarct zone. Six weeks following myocardial infarction, EMAP II protein is elevated above control, changes its location of transcription from the inflammatory cell population to that of the fibroblasts located in the relative avascular scar tissue, and has resumed its perivascular stromal distribution in the viable periinfarct tissue.
Thus, the temporospatial distribution of this antiangiogenic protein suggests that negative vascular modulators may have a function in the revascularization process following acute myocardial infarction.
AuthorsJess L Thompson, James A Ryan, Mark L Barr, Benjamin Franc, Vaughn A Starnes, Margaret A Schwarz
JournalThe Journal of surgical research (J Surg Res) Vol. 116 Issue 1 Pg. 156-64 (Jan 2004) ISSN: 0022-4804 [Print] United States
PMID14732363 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Angiogenesis Inhibitors
  • Cytokines
  • Neoplasm Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • small inducible cytokine subfamily E, member 1
  • Angiogenesis Inhibitors (metabolism)
  • Animals
  • Cytokines (genetics, metabolism)
  • Fibroblasts (metabolism)
  • Male
  • Myocardial Infarction (metabolism, pathology, physiopathology)
  • Myocardium (metabolism)
  • Neoplasm Proteins (genetics, metabolism)
  • Pericytes (metabolism)
  • RNA, Messenger (metabolism)
  • RNA-Binding Proteins (genetics, metabolism)
  • Rats
  • Rats, Sprague-Dawley
  • Tissue Distribution
  • Wound Healing

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: