Abstract | BACKGROUND: METHODS: Spontaneously diabetic NOD mice received NOD severe combined immunodeficiency islet transplants and were treated with daily LSF injections at 50 mg/kg for 3 weeks. Blood glucose levels were monitored. Serum cytokine levels were measured at 1 and 3 weeks after engraftment. Nephrectomy of the islet-implanted kidney was performed in LSF-treated recipients. Histology of islet grafts was assessed at the end of the study. The effect of LSF on beta-cell function was studied in vitro. RESULTS: Without immunosuppressants and insulin, the LSF-treated recipient mice maintained euglycemia significantly longer than the saline-treated recipients (mean, >65 days in the LSF-treated group vs. 6 days in saline controls; P=0.0004). Serum levels of interferon-gamma were markedly reduced in LSF-treated recipients at 1 and 3 weeks posttransplant. Diabetes recurred in the LSF-treated recipients after removing the islet-implanted kidneys. Immunohistochemistry showed retention of insulin-positive cells in the grafts of the LSF-treated recipients. LSF preserved beta-cell insulin secretory function in the presence of inflammatory cytokines in vitro. CONCLUSIONS:
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Authors | Zandong Yang, Meng Chen, Justin D Ellett, Lawrence B Fialkow, Jeffrey D Carter, Jerry L Nadler |
Journal | Transplantation
(Transplantation)
Vol. 77
Issue 1
Pg. 55-60
(Jan 15 2004)
ISSN: 0041-1337 [Print] United States |
PMID | 14724435
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Cytokines
- Inflammation Mediators
- Insulin
- lisofylline
- Pentoxifylline
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Topics |
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(pharmacology)
- Apoptosis
(drug effects)
- Cytokines
(antagonists & inhibitors, classification, metabolism)
- Diabetes Mellitus, Type 1
(prevention & control, surgery)
- Female
- Graft Survival
(drug effects)
- Inflammation Mediators
(antagonists & inhibitors, blood, metabolism)
- Insulin
(metabolism)
- Insulin Secretion
- Islets of Langerhans
(drug effects, metabolism, physiopathology)
- Islets of Langerhans Transplantation
- Mice
- Mice, Inbred NOD
- Mice, SCID
- Pentoxifylline
(analogs & derivatives, pharmacology)
- Secondary Prevention
- Time Factors
- Transplantation, Homologous
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