The mechanism of Polydatin in shock treatment.

Abstract

Polydatin is extracted from a traditional Chinese herbal medicine, Polygonum cuspidatum, and has a special effect in shock treatment. The aim of this study is to explain the cellular and molecular basis of Polydatin in shock treatment. The fluorescent probe techniques, patch clamp method, and cellular flow chamber were used to test intracellular variables of vascular smooth muscle cells (VSMC), myocardial cells (MC), endothelial cells (EC), and white blood cell (WBC). It was shown that Polydatin could inhibit ICAM-1 expression in EC stimulated by lipopolysaccharide (LPS), attenuate WBC-EC adhesion, increase [Ca2+]i in MC with enhancement of MC contraction extent, activate KATP channels of VSMC, and decrease pHi value and [Ca2+]i of VSMC in shock. The study suggests that Polydatin has multiple effects on VSMC, MC, WBC and EC, which are related to the enhancement of heart function and improvement of microcirculatory perfusion in shock.

• Authors: Ke-Seng Zhao, Chunhua Jin, Xuliang Huang, Jie Liu, Wen Sheng Yan, Qiaobing Huang, Wenhong Kan
• Journal: Clinical hemorheology and microcirculation (Clin Hemorheol Microcirc) Vol. 29 Issue 3-4 Pg. 211-7 ( 2003) ISSN: 1386-0291 [Print] Netherlands
• PMID: 14724344 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Calcium Channel Blockers
• Glucosides
• Potassium Channels
• Stilbenes
• Intercellular Adhesion Molecule-1
• Verapamil
• polydatin

Topics

• Animals
• Calcium Channel Blockers (pharmacology)
• Calcium Signaling (drug effects)
• Cell Adhesion (drug effects)
• Cells, Cultured (drug effects)
• Drug Evaluation, Preclinical
• Endothelial Cells (drug effects)
• Endothelium, Vascular (cytology, drug effects)
• Fallopia japonica (chemistry)
• Glucosides (pharmacology, therapeutic use)
• Humans
• Intercellular Adhesion Molecule-1 (biosynthesis, genetics)
• Leukocytes (drug effects, physiology)
• Microcirculation (drug effects)
• Muscle, Smooth, Vascular (cytology, drug effects)
• Myocardium (cytology)
• Patch-Clamp Techniques
• Phytotherapy
• Plants, Medicinal (chemistry)
• Potassium Channels (drug effects)
• Rats
• Shock, Hemorrhagic (drug therapy)
• Shock, Septic (drug therapy, physiopathology)
• Stilbenes (pharmacology, therapeutic use)
• Verapamil (pharmacology)