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Expression of adrenomedullin in hypoxic and ischemic rat kidneys and human kidneys with arterial stenosis.

Abstract
To investigate regional aspects of hypoxic regulation of adrenomedullin (AM) in kidneys, we mapped the distribution of AM in the rat kidney after hypoxia (normobaric hypoxic hypoxia, carbon monoxide, and CoCl(2) for 6 h), anemia (hematocrit lowered by bleeding) and after global transient ischemia for 1 h (unilateral renal artery occlusion and reperfusion for 6 and 24 h) and segmental infarct (6 and 24 h). AM expression and localization was determined in normal human kidneys and in kidneys with arterial stenosis. Hypoxia stimulated AM mRNA expression significantly in rat inner medulla (CO 13 times, 8% O(2) 6 times, and CoCl(2) 8 times), followed by the outer medulla and cortex. AM mRNA level was significantly elevated in response to anemia and occlusion-reperfusion. Immunoreactive AM was associated with the thin limbs of Henle's loop, distal convoluted tubule, collecting ducts, papilla surface epithelium, and urothelium. AM labeling was prominent in the inner medulla after CO and in the outer medulla after occlusion-reperfusion. The infarct border zone was strongly labeled for AM. In cultured inner medullary collecting duct cells, AM mRNA was significantly increased by hypoxia. AM mRNA was equally distributed in human kidney and AM was localized as in the rat kidney. In human kidneys with artery stenosis, AM mRNA was not significantly enhanced compared with controls, but AM immunoreactivity was observed in tubules, vessels, and glomerular cells. In summary, AM expression was increased in the rat kidney in response to hypoxic and ischemic hypoxia in keeping with oxygen gradients. AM was widely distributed in the human kidney with arterial stenosis. AM may play a significant role to counteract hypoxia in the kidney.
AuthorsPeter Sandner, Karl Heinz Hofbauer, Hanna Tinel, Armin Kurtz, Helle C Thiesson, Peter D Ottosen, Steen Walter, Ole Skøtt, Boye L Jensen
JournalAmerican journal of physiology. Regulatory, integrative and comparative physiology (Am J Physiol Regul Integr Comp Physiol) Vol. 286 Issue 5 Pg. R942-51 (May 2004) ISSN: 0363-6119 [Print] United States
PMID14715486 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Peptides
  • RNA, Messenger
  • Adrenomedullin
Topics
  • Adrenomedullin
  • Anemia (metabolism, physiopathology)
  • Animals
  • Cells, Cultured
  • Gene Expression
  • Humans
  • Hypertension, Renal (metabolism, physiopathology)
  • Hypoxia (metabolism, physiopathology)
  • Immunohistochemistry
  • Ischemia (metabolism, physiopathology)
  • Kidney (blood supply, cytology, physiology)
  • Male
  • Peptides (genetics, metabolism)
  • RNA, Messenger (analysis)
  • Rats
  • Rats, Sprague-Dawley
  • Renal Artery Obstruction (metabolism, physiopathology)

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