RNA editing and alternative splicing of human serotonin 2C receptor in schizophrenia.

Abstract

Serotonin 2C receptor (5-HT2CR) heterogeneity in the brain occurs mostly from two different sources: (i) 5-HT2CR mRNA undergoes adenosine-to-inosine editing events at five positions, which leads to amino acid substitutions that produce receptor variants with different pharmacological properties; (ii) 5-HT2CR mRNA is alternatively spliced, resulting in a truncated mRNA isoform (5-HT2CR-tr) which encodes a non-functional serotonin receptor. 5-HT2CR mRNA editing efficiencies and the expression of the full-length and the truncated 5-HT2CR mRNA splice isoforms were analyzed in the prefrontal cortex of elderly subjects with schizophrenia vs. matched controls (ns = 15). No significant differences were found, indicating that there are no alterations in editing or alternative splicing of 5-HT2CRs that are associated with schizophrenia in persons treated with antipsychotic medications. Quantitation of 5-HT2CR and 5-HT2CR-tr mRNA variants revealed that the expression of 5-HT2CR-tr was approximately 50% of that observed for the full-length isoform.

• Authors: Stella Dracheva, Sharif L Elhakem, Sue M Marcus, Larry J Siever, Susan R McGurk, Vahram Haroutunian
• Journal: Journal of neurochemistry (J Neurochem) Vol. 87 Issue 6 Pg. 1402-12 (Dec 2003) ISSN: 0022-3042 [Print] England
• PMID: 14713296 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Protein Isoforms
• RNA, Messenger
• Receptor, Serotonin, 5-HT2C
• Inosine
• Adenosine

Topics

• Adenosine (genetics)
• Aged
• Alternative Splicing
• Case-Control Studies
• Female
• Humans
• Inosine (genetics)
• Male
• Prefrontal Cortex (metabolism)
• Protein Isoforms (genetics, metabolism)
• RNA Editing
• RNA, Messenger (metabolism)
• Receptor, Serotonin, 5-HT2C (genetics)
• Reverse Transcriptase Polymerase Chain Reaction (methods)
• Schizophrenia (genetics, metabolism)