Stimulation of renal Na+ dicarboxylate cotransporter 1 by Na+/H+ exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B.

Abstract	Renal tubular citrate transport is accomplished by electrogenic Na(+) coupled dicarboxylate transporter NaDC-1, a carrier subjected to regulation by acidosis. Trafficking of the Na(+)/H(+) exchanger NHE3 is controlled by NHE regulating factors NHERF-1 and NHERF-2 and the serum and glucocorticoid inducible kinase SGK1. To test for a possible involvement in NaDC-1 regulation, mRNA encoding NaDC-1 was injected into Xenopus oocytes with or without cRNA encoding NHERF-1, NHERF-2, SGK1, SGK2, SGK3, and/or the constitutively active form of the related protein kinase B ((T308,S473D)PKB). Succinate induced inward currents (I(succ)) were taken as a measure of transport rate. Coexpression of neither NHERF-1 nor NHERF-2 in NaDC-1 expressing oocytes significantly altered I(succ). On the other hand, coexpression of SGK1, SGK3, and (T308,S473D)PKB stimulated I(succ), an effect further stimulated by additional coexpression of NHERF-2 but not of NHERF-1. The action of the kinases and NHERF-2 may link urinary citrate excretion to proximal tubular H(+) secretion.
Authors	Christoph Boehmer, Hamdy M Embark, Anna Bauer, Monica Palmada, Chris H Yun, Edward J Weinman, Hitoshi Endou, Philip Cohen, Sven Lahme, Karl-Horst Bichler, Florian Lang
Journal	Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 313 Issue 4 Pg. 998-1003 (Jan 23 2004) ISSN: 0006-291X [Print] United States
PMID	14706641 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Cytoskeletal Proteins Dicarboxylic Acid Transporters Immediate-Early Proteins Nuclear Proteins Organic Anion Transporters, Sodium-Dependent Phosphoproteins Proto-Oncogene Proteins Recombinant Proteins SLC13A2 protein, human Slc13a2 protein, rat Slc9a3r2 protein, rat Sodium-Hydrogen Exchangers Symporters sodium-hydrogen exchanger regulatory factor Citric Acid Succinic Acid Protein Serine-Threonine Kinases Proto-Oncogene Proteins c-akt serum-glucocorticoid regulated kinase
Topics	Animals Citric Acid (metabolism) Cytoskeletal Proteins (genetics, metabolism) Dicarboxylic Acid Transporters (genetics, metabolism) Female Humans Immediate-Early Proteins In Vitro Techniques Kidney (metabolism) Kidney Tubules, Proximal (metabolism) Kinetics Nuclear Proteins Oocytes (drug effects, metabolism) Organic Anion Transporters, Sodium-Dependent (genetics, metabolism) Phosphoproteins (genetics, metabolism) Protein Serine-Threonine Kinases (genetics, metabolism) Proto-Oncogene Proteins (genetics, metabolism) Proto-Oncogene Proteins c-akt Rats Recombinant Proteins (genetics, metabolism) Sodium-Hydrogen Exchangers Succinic Acid (pharmacology) Symporters (genetics, metabolism) Xenopus laevis

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