Abstract | AIM: METHODS: RESULTS: After the gastric cancer cells were exposed to TRAIL 300 ng/ml for 24 hours, the apoptosis rate was 36.05%, 20.27%, 16.50% and 11.80% in MKN28, MKN45, AGS and SGC-7901cells respectively. Western blot revealed that the expressions of NF-kappaB and survivin were lower in MKN28 cells than in MKN45, AGS and SGC-7901 cells. In contrast, the expression of Caspase3 was higher in MKN28 cells than in MKN45, AGS and SGC-7901 cells. CONCLUSION: There is a selectivity of TRAIL potency to induce apoptosis in gastric cancer cells of different cell lines. The anticancer potency of TRAIL is associated with the decreased expression of NF-kappaB and survivin and increased expression of Caspase3 of gastric cancer cells.
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Authors | Liu-Qin Yang, Dian-Chun Fang, Rong-Quan Wang, Shi-Ming Yang |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 10
Issue 1
Pg. 22-5
(Jan 2004)
ISSN: 1007-9327 [Print] United States |
PMID | 14695762
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apoptosis Regulatory Proteins
- BIRC5 protein, human
- Inhibitor of Apoptosis Proteins
- Membrane Glycoproteins
- Microtubule-Associated Proteins
- NF-kappa B
- Neoplasm Proteins
- Proto-Oncogene Proteins c-bcl-2
- Survivin
- TNF-Related Apoptosis-Inducing Ligand
- TNFSF10 protein, human
- Tumor Necrosis Factor-alpha
- CASP3 protein, human
- Caspase 3
- Caspases
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Topics |
- Apoptosis
(drug effects, physiology)
- Apoptosis Regulatory Proteins
- Caspase 3
- Caspases
(metabolism)
- Cell Line, Tumor
- Dose-Response Relationship, Drug
- Humans
- Inhibitor of Apoptosis Proteins
- Membrane Glycoproteins
(pharmacology)
- Microtubule-Associated Proteins
(metabolism)
- NF-kappa B
(metabolism)
- Neoplasm Proteins
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- Stomach Neoplasms
- Survivin
- TNF-Related Apoptosis-Inducing Ligand
- Tumor Necrosis Factor-alpha
(pharmacology)
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