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Effect of NF-kappaB, survivin, Bcl-2 and Caspase3 on apoptosis of gastric cancer cells induced by tumor necrosis factor related apoptosis inducing ligand.

AbstractAIM:
To study the effect of NF-kappaB, survivin, Bcl-2 and Caspase3 on tumor necrosis factors related apoptosis inducing ligand (TRAIL) induced apoptosis of gastric cancer cells.
METHODS:
Gastric cancer cells of SGC-7901, MKN28, MKN45 and AGS lines were cultured in PRMI-1640 medium and the apoptosis rates of the cells of 4 lines were observed after treatment of tumor necrosis factors related apoptosis inducing ligand (TRAIL) with a flow cytometer. The expression of NF-kappaB, survivin, Bcl-2 and Caspase3 in gastric cancer cells of 4 lines was analyzed with Western blot.
RESULTS:
After the gastric cancer cells were exposed to TRAIL 300 ng/ml for 24 hours, the apoptosis rate was 36.05%, 20.27%, 16.50% and 11.80% in MKN28, MKN45, AGS and SGC-7901cells respectively. Western blot revealed that the expressions of NF-kappaB and survivin were lower in MKN28 cells than in MKN45, AGS and SGC-7901 cells. In contrast, the expression of Caspase3 was higher in MKN28 cells than in MKN45, AGS and SGC-7901 cells.
CONCLUSION:
There is a selectivity of TRAIL potency to induce apoptosis in gastric cancer cells of different cell lines. The anticancer potency of TRAIL is associated with the decreased expression of NF-kappaB and survivin and increased expression of Caspase3 of gastric cancer cells.
AuthorsLiu-Qin Yang, Dian-Chun Fang, Rong-Quan Wang, Shi-Ming Yang
JournalWorld journal of gastroenterology (World J Gastroenterol) Vol. 10 Issue 1 Pg. 22-5 (Jan 2004) ISSN: 1007-9327 [Print] United States
PMID14695762 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Apoptosis Regulatory Proteins
  • BIRC5 protein, human
  • Inhibitor of Apoptosis Proteins
  • Membrane Glycoproteins
  • Microtubule-Associated Proteins
  • NF-kappa B
  • Neoplasm Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Survivin
  • TNF-Related Apoptosis-Inducing Ligand
  • TNFSF10 protein, human
  • Tumor Necrosis Factor-alpha
  • CASP3 protein, human
  • Caspase 3
  • Caspases
Topics
  • Apoptosis (drug effects, physiology)
  • Apoptosis Regulatory Proteins
  • Caspase 3
  • Caspases (metabolism)
  • Cell Line, Tumor
  • Dose-Response Relationship, Drug
  • Humans
  • Inhibitor of Apoptosis Proteins
  • Membrane Glycoproteins (pharmacology)
  • Microtubule-Associated Proteins (metabolism)
  • NF-kappa B (metabolism)
  • Neoplasm Proteins
  • Proto-Oncogene Proteins c-bcl-2 (metabolism)
  • Stomach Neoplasms
  • Survivin
  • TNF-Related Apoptosis-Inducing Ligand
  • Tumor Necrosis Factor-alpha (pharmacology)

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