[Study on the therapeutic effect of combined use of obidoxime and atropine with respiratory machine on respiratory muscle paralysis caused by omethoate poisoning of rats].

Abstract

OBJECTIVE:

To examine the therapeutic effect of combined use of obidoxime and atropine with artificial ventilation on respiratory muscle paralysis caused by omethoate poisoning in rats.

METHODS:

Rats were exposed to 2 times the dose of LD50 omethoate and treated with atropine (10 mg/kg) to counteract cholinergic symptoms. When the rats' respiratory frequency became slower and breathed with difficulty, the trachea intubation and artificial ventilation was carried out. The rats in group A were continuously treated with atropine. The dose of obidoxime for Group B, C and D were 8, 15, 20 mg/kg respectively, given at the same time as artificial ventilation and 1, 2, 3 hours later. The doses of atropine was reduced to 1/3 - 2/3 of the first dose so as to maintain the rats atropinized. If the rat survival was beyond 60 minutes after withdrawal of artificial ventilation, the combined treatment was considered successful. The function of isolated phrenic diaphragm of the rats was observed with MS-302 physiological and pharmacological analysis instrument.

RESULTS:

None of the rats in Group A was successful after withdrawal from artificial ventilation and the function of phrenic diaphragm appeared poor; whereas > 80% of the rats in B, C, D Group were successful after withdrawal from artificial ventilation in 3 h and the function of phrenic diaphragm remained well. The survival rate in B, C and D groups were higher after withdrawal from artificial ventilation than that in Group A(P < 0.01). The function of phrenic diaphragm in Group B, C and D were gradually decreased after ACh was added into the container.

CONCLUSIONS:

Combined use of suitable dose of obidoxime and atropine with artificial ventilation for respiratory muscle paralysis caused by omethoate poisoning could promote the recovery of diaphragm function and reduce the death rate in poisoned rats.

• Authors: Zewu Qiu, Delu Zhao, Yinkui Shi, Shaoqing Huang
• Journal: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases (Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi) Vol. 20 Issue 5 Pg. 359-61 (Oct 2002) ISSN: 1001-9391 [Print] China
• PMID: 14694726 (Publication Type: English Abstract, Journal Article)

Chemical References

• dimethoxon
• Obidoxime Chloride
• Atropine
• Dimethoate

Topics

• Animals
• Atropine (administration & dosage)
• Dimethoate (analogs & derivatives, poisoning)
• Drug Therapy, Combination
• Obidoxime Chloride (administration & dosage)
• Rats
• Respiration, Artificial
• Respiratory Paralysis (drug therapy)