[Effects of lanthanum chloride on the apoptosis of fibroblasts in trauma tissue].

Abstract

OBJECTIVE:

To investigate the effects of lanthanum chloride on the apoptosis of fibroblasts in trauma tissue.

METHODS:

Fifty adult female SD rats were used and linear incisions were made on the back near the joints of extremities of the rats. One of the cuts receiving no treatment was designated as blank control (C). 0.25 ml of distilled water, lanthanum chloride (50 mmol/L) and the antibody of (TGFbeta(1)) transforming growth factor beta(1) (0.2 mg/ml) were respectively injected into the both sides of the other three wounds subcutaneously and the wounds were divided into simulating control (SC), lanthanum chloride (LC) and antibody (A) groups. The fibroblast apoptosis in the wound tissue samples and the change in intracellular calcium concentration (Ca(2+)) were determined by flow cytometry (FCM) and TUNEL methods on the 14th and 28th day after the injection.

RESULTS:

Apoptosis of fibroblasts was enhanced significantly after 14 days of injection in LC and A groups compared with that in C and SC groups (P < 0.05 approximately 0.01). Furthermore, intracellular Ca(2+) was increased evidently in LC group (P < 0.01).

CONCLUSION:

It is indicated that lanthanum chloride might be effective in preventing scar development.

• Authors: Xiao-Chun Zhong, Yu-Cheng Dai, Yong Cao, Guo-Hui Li, Jie Li, Xie-Qing Wu, Qiang Liu
• Journal: Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns (Zhonghua Shao Shang Za Zhi) Vol. 19 Issue 5 Pg. 297-9 (Oct 2003) ISSN: 1009-2587 [Print] China
• PMID: 14687535 (Publication Type: English Abstract, Journal Article)

Chemical References

• lanthanum chloride
• Lanthanum
• Calcium

Topics

• Animals
• Apoptosis (drug effects)
• Calcium (metabolism)
• Cicatrix (prevention & control)
• Female
• Fibroblasts (drug effects, pathology)
• Flow Cytometry
• Lanthanum (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Wound Healing (drug effects)