Production and characterisation of monoclonal antibodies to ovine interleukin-5.

Abstract

Monoclonal antibodies (MAbs) were developed against recombinant ovine interleukin-5 (IL-5) produced in the baculovirus expression vector system. One MAb, D11 (isotype IgG1), neutralised the activity of both recombinant and native sources of IL-5 in a biological assay (Baf cell assay) but was only weakly reactive in immunocytochemistry. Conversely, a second MAb, A8 (isotype IgA), successfully detected IL-5 in immunocytochemistry but did not display neutralising activity. The development of these MAbs will enable the assay of ovine IL-5 in vitro and permit studies into the role of hypersensitivity reactions in sheep by neutralisation of IL-5 in vivo.

• Authors: Tuan Bendixsen, Katrina L Bosward, David L Emery
• Journal: Parasitology international (Parasitol Int) Vol. 52 Issue 4 Pg. 281-90 (Dec 2003) ISSN: 1383-5769 [Print] Netherlands
• PMID: 14665384 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens, Helminth
• Interleukin-5
• Recombinant Proteins

Topics

• Animals
• Antibodies, Monoclonal
• Antigens, Helminth (immunology)
• Baculoviridae (genetics)
• CHO Cells
• Cricetinae
• Genetic Vectors
• Interleukin-5 (blood, immunology)
• Recombinant Proteins (immunology)
• Sheep
• Transfection
• Trichostrongylus (immunology)