Production and characterisation of monoclonal antibodies to ovine interleukin-5.

Monoclonal antibodies (MAbs) were developed against recombinant ovine interleukin-5 (IL-5) produced in the baculovirus expression vector system. One MAb, D11 (isotype IgG1), neutralised the activity of both recombinant and native sources of IL-5 in a biological assay (Baf cell assay) but was only weakly reactive in immunocytochemistry. Conversely, a second MAb, A8 (isotype IgA), successfully detected IL-5 in immunocytochemistry but did not display neutralising activity. The development of these MAbs will enable the assay of ovine IL-5 in vitro and permit studies into the role of hypersensitivity reactions in sheep by neutralisation of IL-5 in vivo.
AuthorsTuan Bendixsen, Katrina L Bosward, David L Emery
JournalParasitology international (Parasitol Int) Vol. 52 Issue 4 Pg. 281-90 (Dec 2003) ISSN: 1383-5769 [Print] Netherlands
PMID14665384 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Monoclonal
  • Antigens, Helminth
  • Interleukin-5
  • Recombinant Proteins
  • Animals
  • Antibodies, Monoclonal
  • Antigens, Helminth (immunology)
  • Baculoviridae (genetics)
  • CHO Cells
  • Cricetinae
  • Genetic Vectors
  • Interleukin-5 (blood, immunology)
  • Recombinant Proteins (immunology)
  • Sheep
  • Transfection
  • Trichostrongylus (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: