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Production and characterisation of monoclonal antibodies to ovine interleukin-5.

Abstract
Monoclonal antibodies (MAbs) were developed against recombinant ovine interleukin-5 (IL-5) produced in the baculovirus expression vector system. One MAb, D11 (isotype IgG1), neutralised the activity of both recombinant and native sources of IL-5 in a biological assay (Baf cell assay) but was only weakly reactive in immunocytochemistry. Conversely, a second MAb, A8 (isotype IgA), successfully detected IL-5 in immunocytochemistry but did not display neutralising activity. The development of these MAbs will enable the assay of ovine IL-5 in vitro and permit studies into the role of hypersensitivity reactions in sheep by neutralisation of IL-5 in vivo.
AuthorsTuan Bendixsen, Katrina L Bosward, David L Emery
JournalParasitology international (Parasitol Int) Vol. 52 Issue 4 Pg. 281-90 (Dec 2003) ISSN: 1383-5769 [Print] Netherlands
PMID14665384 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Monoclonal
  • Antigens, Helminth
  • Interleukin-5
  • Recombinant Proteins
Topics
  • Animals
  • Antibodies, Monoclonal
  • Antigens, Helminth (immunology)
  • Baculoviridae (genetics)
  • CHO Cells
  • Cricetinae
  • Genetic Vectors
  • Interleukin-5 (blood, immunology)
  • Recombinant Proteins (immunology)
  • Sheep
  • Transfection
  • Trichostrongylus (immunology)

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