Effects of vitamin E and electrical stimulation on the denervated rat gastrocnemius muscle malondialdehyde and glutathione levels.

Abstract

Effects of vitamin E treatment and local electrical stimulation on progression of atrophy in the denervated rat gastrocnemius muscle were studied. Denervation was performed by right leg sciatic nerve axotomy. Electrical stimulation (3-10 mA ms(-1), 10 min per day for 7 days) was applied to the right gastrocnemius muscle starting from day 1 of denervation. The muscle samples were assayed for malondialdehyde (MDA) and glutathione levels, as well as the histological appearance after 8 days of denervation. MDA levels were markedly increased following denervation. However, electrical stimulation, vitamin E treatment (30 mg kg(-1), i.m., everyday for 7 days), and combination of electrical stimulation and vitamin E treatment markedly reduced MDA levels. Glutathione levels were significantly decreased in the denervation group. Electrical stimulation, vitamin E treatment, and electrical stimulation plus vitamin E treatment prevented these reductions in glutathione levels. In the vitamin E treatment group, glutathione levels were markedly higher than in the control group. These results indicate that electrical stimulation and vitamin E treatment alone, or in combination, were able to prevent the effects of denervation on muscle atrophy.

• Authors: Seniz Demiryürek, Aydan Babül
• Journal: The International journal of neuroscience (Int J Neurosci) Vol. 114 Issue 1 Pg. 45-54 (Jan 2004) ISSN: 0020-7454 [Print] England
• PMID: 14660066 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Vitamin E
• Malondialdehyde
• Glutathione

Topics

• Analysis of Variance
• Animals
• Electric Stimulation (methods)
• Glutathione (metabolism)
• Malondialdehyde (metabolism)
• Muscle, Skeletal (drug effects, metabolism, pathology, radiation effects)
• Parasympathectomy
• Random Allocation
• Rats
• Rats, Wistar
• Sciatic Nerve (surgery)
• Vitamin E (pharmacology)