HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Cytokinin-induced differentiation of human myeloid leukemia HL-60 cells is associated with the formation of nucleotides, but not with incorporation into DNA or RNA.

Abstract
Cytokinins are important purine derivatives that act as hormones to control many processes in plants. Cytokinins such as isopentenyladenine (IPA), kinetin and benzyladenine were very effective at inducing the granulocytic differentiation of human myeloid leukemia HL-60 cells. The metabolism of cytokinins to their nucleotides was closely associated with cytokinin-induced differentiation and growth inhibition. When the cells were incubated with [14C]-benzyladenine, radioactivity was significantly incorporated into RNA and DNA. However, the radioactive nucleotides in RNA or DNA were adenine nucleotides, not benzyladenine nucleotides, suggesting that cytokinins were not incorporated into RNA and DNA. The benzyladenine nucleotides were not stably released into the medium in intact form. Cytokinins effectively induced a phosphorylated (active) form of mitogen-activated protein kinase (MAPK). MAPK activation was necessary for cytokinin-induced differentiation, because PD98059, an inhibitor of MAPK kinase, suppressed the differentiation induced by cytokinins. These results suggest that cytokinin nucleotides themselves play an important role in inducing the differentiation of HL-60 cells.
AuthorsYuki Ishii, Shingo Sakai, Yoshio Honma
JournalBiochimica et biophysica acta (Biochim Biophys Acta) Vol. 1643 Issue 1-3 Pg. 11-24 (Dec 07 2003) ISSN: 0006-3002 [Print] Netherlands
PMID14654224 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Cytokinins
  • Nucleotides
  • Radioactive Tracers
  • RNA
  • 3-benzyladenine
  • DNA
  • Mitogen-Activated Protein Kinases
  • Adenine
Topics
  • Adenine (analogs & derivatives, metabolism)
  • Cell Differentiation (drug effects)
  • Cell Division (drug effects)
  • Cytokinins (metabolism, pharmacology)
  • DNA (biosynthesis)
  • HL-60 Cells
  • Humans
  • Leukemia, Myeloid (pathology)
  • Mitogen-Activated Protein Kinases (metabolism)
  • Nucleotides (biosynthesis)
  • Phosphorylation
  • RNA (biosynthesis)
  • Radioactive Tracers

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: