| Abstract | Propolis is a bee product, which has long been used in folk medicine for the management of different diseases. In this study we evaluated the analgesic and anti-inflammatory effects of a standard ethanolic extract of Bulgarian propolis (Et-Blg) in mice and its in vitro effect on airway smooth muscle. Et-Blg inhibited acetic acid-induced abdominal contortions with an ID(50) = 7.4 +/- 0.7 mg. kg(-1). In the formalin test, the extract caused a significant reduction in pain in mice treated with 100 mg. kg(-1) Et-Blg during the neurogenic phase and for the inflammatory phase with all doses of the extract, with an ID(50) = 2.5 +/- 0.4 mg. kg(-1). Et-Blg inhibited also the capsaicin-induced ear edema in mice; however, this extract was ineffective when assessed in the tail-flick and hot-plate thermal assays. The analgesic effect of Et-Blg was associated with the inhibition of inflammatory responses and not to a simple irritation of nervous terminals. In vitro, this extract inhibited the contraction of trachea smooth muscle induced by histamine (IC(50) = 50 +/- 5 microg. mL(-1)), capsaicin (IC(50) = 26.8 +/- 3 microg. mL(-1)), 80 mM KCl (IC(50) = 27.8 +/- 3 microg. mL(-1)), and carbachol (IC(50) = 54 +/- 2 microg. mL(-1)). |
| Authors | Niraldo Paulino, Andreia Pires Dantas, Vassya Bankova, Daniela Taggliari Longhi, Amarilis Scremin, Solange Lisboa de Castro, João Batista Calixto
(Affiliation: Grupo de Pesquisa e Desenvolvimento de Biofármacos (BIOFAR), Universidade do Sul de Santa Catarina, Tubarão, SC, Brazil.)
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| Journal | Journal of pharmacological sciences
(J Pharmacol Sci)
Vol. 93
Issue 3
Pg. 307-13
(Nov 2003)
ISSN: 1347-8613 Japan |
| PMID | 14646248
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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| Chemical References |
- Analgesics, Non-Narcotic
- Anti-Inflammatory Agents, Non-Steroidal
- Propolis
|
| Topics |
- Analgesics, Non-Narcotic
(isolation & purification, pharmacology, therapeutic use)
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(isolation & purification, pharmacology, therapeutic use)
- Bulgaria
- Dose-Response Relationship, Drug
- Guinea Pigs
- Male
- Mice
- Muscle Contraction
(drug effects, physiology)
- Muscle, Smooth
(drug effects, physiology)
- Pain Measurement
(drug effects, methods)
- Propolis
(isolation & purification, pharmacology, therapeutic use)
- Trachea
(drug effects, physiology)
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