Anti-cardiolipin and anti-beta2-glycoprotein I (beta2GP-I) antibody assays as screening for anti-phospholipid syndrome.

Abstract

BACKGROUND:

Antiphospholipid antibodies are a family of autoantibodies that exhibit a broad range of target specificities and affinities, all recognizing various combinations of phospholipids, phospholipid binding proteins or both.

OBJECTIVE:

To evaluate the frequency of anticardiolipin (aCL) or anti-beta-2 glycoprotein I (anti-beta2GPI) antibodies in a cohort of patients with primary (PAPS) and secondary (SAPS) antiphospholipid syndrome and other rheumatic and infectious conditions.

METHODS:

Sera were drawn from 226 patients with PAPS (n= 66), SAPS (n= 60), rheumatoid arthritis (RA) (n= 30), scleroderma (SSc) (n= 30) or syphilis (n= 30). IgG, IgM, IgA aCL and anti-beta2GPI antibodies were determined for all patients. We employed Varelisa diagnostic kits (Pharmacia Diagnostics GmbH & Co.KG, Germany).

RESULTS:

In APS patients: aCL and anti-betaGP1 antibodies were detected in 81.8% and 70% in PAPS and SAPS patients, respectively. In PAPS, aCL were detected in 71.2%, and anti-beta2GP1 in 50% of patients. In SAPS, aCL were detected in 63.3%, and anti-beta2GP1 in 53.3% of patients. In syphilis, aCL and anti-beta2GP1 antibodies were detected in 46.7% of patients in low levels: aCL in 36.7% and anti-beta2GP1 in 20% of patients. aCL antibodies were detected in 10%, 13.3%, and 0% of RA patients, SSc patients, and healthy persons, respectively. Anti-beta2GP1 antibodies were not detected in RA patients, SSc patients, and healthy persons.

CONCLUSIONS:

aCL and anti-beta2GP1 antibodies are more frequently found in sera of APS patients, and can be found in low levels in syphilis. Anti-beta2GP1 assay found to be more specific than aCL in antiphospholipid antibodies determination.

• Authors: I Marai, B Gilburd, M Blank, Y Shoenfeld
• Journal: Human antibodies (Hum Antibodies) Vol. 12 Issue 3 Pg. 57-62 ( 2003) ISSN: 1093-2607 [Print] Netherlands
• PMID: 14646033 (Publication Type: Journal Article)

Chemical References

• Autoantibodies
• Cardiolipins
• Glycoproteins
• beta 2-Glycoprotein I

Topics

• Antiphospholipid Syndrome (blood, diagnosis, immunology)
• Arthritis, Rheumatoid (immunology)
• Autoantibodies (immunology)
• Cardiolipins (immunology)
• Cohort Studies
• Glycoproteins (immunology)
• Humans
• Scleroderma, Systemic (immunology)
• Syphilis (immunology)
• beta 2-Glycoprotein I