Abstract |
Cytomegaloviruses encode homologs of cellular immune effector proteins, including chemokines (CKs) and CK receptor-like G protein-coupled receptors (GPCRs). Sequence of the guinea pig cytomegalovirus (GPCMV) genome identified an open reading frame (ORF) which predicted a 101 amino acid (aa) protein with homology to the macrophage inflammatory protein (MIP) subfamily of CC (beta) CKs, designated GPCMV-MIP. To assess functionality of this CK, recombinant GPCMV-MIP was expressed in HEK293 cells and assayed for its ability to bind to and functionally interact with a variety of GPCRs. Specific signaling was observed with the hCCR1 receptor, which could be blocked with hMIP -1alpha in competition experiments. Migration assays revealed that GPCMV-MIP was able to induce chemotaxis in hCCR1-L1.2 cells. Antisera raised against a GST-MIP fusion protein immunoprecipitated species of approximately 12 and 10 kDa from GPCMV-inoculated tissue culture lysates, and convalescent antiserum from GPCMV-infected animals was immunoreactive with GST-MIP by ELISA assay. These results represent the first substantive in vitro characterization of a functional CC CK encoded by a cytomegalovirus.
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Authors | Mark Penfold, Zhenhua Miao, Yu Wang, Shannon Haggerty, Mark R Schleiss |
Journal | Virology
(Virology)
Vol. 316
Issue 2
Pg. 202-12
(Nov 25 2003)
ISSN: 0042-6822 [Print] United States |
PMID | 14644603
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Macrophage Inflammatory Proteins
- Receptors, CCR1
- Receptors, Chemokine
- Viral Proteins
- Calcium
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Topics |
- Amino Acid Sequence
- Animals
- Calcium
(metabolism)
- Cell Movement
- Guinea Pigs
- Macrophage Inflammatory Proteins
(chemistry, genetics, physiology)
- Molecular Sequence Data
- Receptors, CCR1
- Receptors, Chemokine
(physiology)
- Roseolovirus
(genetics, immunology)
- Signal Transduction
(physiology)
- Viral Proteins
(chemistry, genetics, physiology)
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