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A macrophage inflammatory protein homolog encoded by guinea pig cytomegalovirus signals via CC chemokine receptor 1.

Abstract
Cytomegaloviruses encode homologs of cellular immune effector proteins, including chemokines (CKs) and CK receptor-like G protein-coupled receptors (GPCRs). Sequence of the guinea pig cytomegalovirus (GPCMV) genome identified an open reading frame (ORF) which predicted a 101 amino acid (aa) protein with homology to the macrophage inflammatory protein (MIP) subfamily of CC (beta) CKs, designated GPCMV-MIP. To assess functionality of this CK, recombinant GPCMV-MIP was expressed in HEK293 cells and assayed for its ability to bind to and functionally interact with a variety of GPCRs. Specific signaling was observed with the hCCR1 receptor, which could be blocked with hMIP -1alpha in competition experiments. Migration assays revealed that GPCMV-MIP was able to induce chemotaxis in hCCR1-L1.2 cells. Antisera raised against a GST-MIP fusion protein immunoprecipitated species of approximately 12 and 10 kDa from GPCMV-inoculated tissue culture lysates, and convalescent antiserum from GPCMV-infected animals was immunoreactive with GST-MIP by ELISA assay. These results represent the first substantive in vitro characterization of a functional CC CK encoded by a cytomegalovirus.
AuthorsMark Penfold, Zhenhua Miao, Yu Wang, Shannon Haggerty, Mark R Schleiss
JournalVirology (Virology) Vol. 316 Issue 2 Pg. 202-12 (Nov 25 2003) ISSN: 0042-6822 [Print] United States
PMID14644603 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Macrophage Inflammatory Proteins
  • Receptors, CCR1
  • Receptors, Chemokine
  • Viral Proteins
  • Calcium
Topics
  • Amino Acid Sequence
  • Animals
  • Calcium (metabolism)
  • Cell Movement
  • Guinea Pigs
  • Macrophage Inflammatory Proteins (chemistry, genetics, physiology)
  • Molecular Sequence Data
  • Receptors, CCR1
  • Receptors, Chemokine (physiology)
  • Roseolovirus (genetics, immunology)
  • Signal Transduction (physiology)
  • Viral Proteins (chemistry, genetics, physiology)

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