3-Nitrobenzanthrone (3-NBA) induced micronucleus formation and DNA damage in human hepatoma (HepG2) cells.

Abstract

3-Nitrobenzanthrone (3-NBA), identified in diesel exhaust and in airborne particulate matter, is a potent mutagen in Salmonella, induces micronuclei formation in mice and in human cells and DNA adducts in rats. In the present study, we investigated the genotoxic potency of 3-NBA in human HepG2 cells using the micronucleus (MN) assay and the single cell gel electrophoresis (SCGE). 3-NBA caused a genotoxic effect at concentrations > or =12 nM in both assays. In the micronucleus assay, we found 98.7+/-10.3 MN/1000 BNC at a concentration of 100 nM 3-NBA in comparison to 27.3+/-0.6 MN/1000 BNC with the negative control. At the same concentration, the DNA-migration (SCGE) showed an Olive tail moment (OTM) of 2.7+/-0.45 and %DNA in the tail of 8.28+/-0.76; OTM and %DNA in the tail of cells treated with the negative control were 0.73+/-0.08 and 2.81+/-0.30, respectively. The results are discussed under consideration of former studies.

• Authors: Evelyn Lamy, Fekadu Kassie, Richard Gminski, Heinz H Schmeiser, Volker Mersch-Sundermann
• Journal: Toxicology letters (Toxicol Lett) Vol. 146 Issue 2 Pg. 103-9 (Jan 15 2004) ISSN: 0378-4274 [Print] Netherlands
• PMID: 14643962 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benz(a)Anthracenes
• 3-nitrobenzanthrone

Topics

• Benz(a)Anthracenes (toxicity)
• Carcinoma, Hepatocellular (pathology)
• Cell Line, Tumor
• Comet Assay
• DNA Damage
• Dose-Response Relationship, Drug
• Humans
• Liver Neoplasms (pathology)
• Micronuclei, Chromosome-Defective (drug effects)