Abstract |
3-Nitrobenzanthrone (3-NBA), identified in diesel exhaust and in airborne particulate matter, is a potent mutagen in Salmonella, induces micronuclei formation in mice and in human cells and DNA adducts in rats. In the present study, we investigated the genotoxic potency of 3-NBA in human HepG2 cells using the micronucleus (MN) assay and the single cell gel electrophoresis (SCGE). 3-NBA caused a genotoxic effect at concentrations > or =12 nM in both assays. In the micronucleus assay, we found 98.7+/-10.3 MN/1000 BNC at a concentration of 100 nM 3-NBA in comparison to 27.3+/-0.6 MN/1000 BNC with the negative control. At the same concentration, the DNA-migration (SCGE) showed an Olive tail moment (OTM) of 2.7+/-0.45 and % DNA in the tail of 8.28+/-0.76; OTM and % DNA in the tail of cells treated with the negative control were 0.73+/-0.08 and 2.81+/-0.30, respectively. The results are discussed under consideration of former studies.
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Authors | Evelyn Lamy, Fekadu Kassie, Richard Gminski, Heinz H Schmeiser, Volker Mersch-Sundermann |
Journal | Toxicology letters
(Toxicol Lett)
Vol. 146
Issue 2
Pg. 103-9
(Jan 15 2004)
ISSN: 0378-4274 [Print] Netherlands |
PMID | 14643962
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Benz(a)Anthracenes
- 3-nitrobenzanthrone
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Topics |
- Benz(a)Anthracenes
(toxicity)
- Carcinoma, Hepatocellular
(pathology)
- Cell Line, Tumor
- Comet Assay
- DNA Damage
- Dose-Response Relationship, Drug
- Humans
- Liver Neoplasms
(pathology)
- Micronuclei, Chromosome-Defective
(drug effects)
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