Abstract | BACKGROUND: METHODS: The method is based on the use of alditol acetate derivatives giving a lower number of GC peaks than reducing sugars do. Acetate derivatives are more stable and less expensive than GC silylates and keep the flame-detector cleaner. We checked the chemical stability of alditol acetates by verifying the reproducibility of the standard curve of a sugar derivative sample which had been stored for 2 months at -20 degrees C. RESULTS: CONCLUSIONS: A fast, reliable and cheap gas-chromatographic method is presented here to evaluate gastric and intestinal permeability.
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Authors | Cristiana Abazia, Renata Ferrara, Maria Michela Corsaro, Gaspare Barone, Pietro Coccoli, Gianpaolo Parrilli |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 338
Issue 1-2
Pg. 25-32
(Dec 2003)
ISSN: 0009-8981 [Print] Netherlands |
PMID | 14637262
(Publication Type: Journal Article)
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Chemical References |
- Lactulose
- Sucrose
- Rhamnose
|
Topics |
- Celiac Disease
(diagnosis, physiopathology, urine)
- Chromatography, Gas
- Digestive System
(metabolism, physiopathology)
- Female
- Humans
- Intestinal Absorption
- Lactulose
(urine)
- Male
- Permeability
- Rhamnose
(urine)
- Sensitivity and Specificity
- Sucrose
(urine)
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