Polyion complex micelles entrapping cationic dendrimer porphyrin: effective photosensitizer for photodynamic therapy of cancer.

Abstract

Photosensitizers play a crucial role in the photodynamic therapy (PDT) of cancer. In this study, a third-generation aryl ether dendrimer porphyrin with 32 primary amine groups on the periphery, [NH2CH2CH2NHCO]32DPZn, and pH-sensitive, polyion complex micelles (PIC) composed of the porphyrin dendrimer and PEG-b-poly(aspartic acid), were evaluated as new photosensitizers (PSs) for PDT in the Lewis Lung Carcinoma (LLC) cell line. The preliminary photophysical characteristics of [NH2CH2CH2NHCO]32DPZn and the corresponding micelles were investigated. Electrostatic assembly resulted in a red-shift of the Soret peak of the porphyrin core and the enhanced fluorescence. Compared to the dendrimer porphyrin [NH2CH2CH2NHCO]32DPZn, relatively low cellular uptake of dendrimer porphyrin [NH2CH2CH2NHCO]32DPZn incorporated in the PIC micelle was observed, yet the latter exhibited enhanced photodynamic efficacy on the LLC cell line. Importantly, the use of PIC micelles as a delivery system reduced the dark toxicity of the cationic dendrimer porphyrin, probably due to the biocompatible PEG shell of the micelles.

• Authors: Guo-Dong Zhang, Atsushi Harada, Nobuhiro Nishiyama, Dong-Lin Jiang, Hiroyuki Koyama, Takuzo Aida, Kazunori Kataoka
• Journal: Journal of controlled release : official journal of the Controlled Release Society (J Control Release) Vol. 93 Issue 2 Pg. 141-50 (Dec 05 2003) ISSN: 0168-3659 [Print] Netherlands
• PMID: 14636720 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drug Carriers
• Macromolecular Substances
• Metalloporphyrins
• Micelles
• Phenyl Ethers
• Photosensitizing Agents
• Polymers
• Porphyrins
• Proteins
• polyethylene glycol-block-polyaspartic acid
• Aspartic Acid
• Polyethylene Glycols

Topics

• Animals
• Aspartic Acid (chemistry)
• Carcinoma, Lewis Lung (pathology)
• Drug Carriers (pharmacology)
• Forecasting
• Macromolecular Substances
• Metalloporphyrins (chemical synthesis, metabolism, pharmacology)
• Micelles
• Nanotechnology
• Neoplasms (therapy)
• Phenyl Ethers (chemical synthesis, metabolism, pharmacology)
• Photochemotherapy (methods)
• Photosensitizing Agents (chemical synthesis, pharmacology, therapeutic use)
• Phototherapy
• Polyethylene Glycols (chemistry, pharmacology)
• Polymers
• Porphyrins (chemistry)
• Proteins (chemistry, pharmacology)
• Spectrometry, Fluorescence
• Tumor Cells, Cultured