PMN/platelets coinfused in guinea pig hearts Exposed to low-flow ischemia have no additive cardiodepressive effect.

Abstract

Recent studies revealed an additive cardiodepressive effect of polymorphonuclear granulocytes (PMN) and thrombocytes in hearts exposed to a no-flow ischemia. To find out whether or not this is also true for isolated guinea pig hearts exposed to a low-flow ischemia, the current study was performed. PMN or thrombocytes, together or separately, were applied as a 1-min bolus (1,000/microl or 20,000/microl, respectively) during ischemia or in reperfusion in the presence of thrombin (0.3 U/ml perfusate). Recovery of external heart work and intracoronary cell retention were quantified in percent. Sole application of PMN or platelets during ischemia and reperfusion significantly compromised myocardial function, whereas coapplication of PMN and platelets did not exhibit any further cardiodepressive effect. Coapplication of cells almost prevented intracoronary platelet retention during ischemia and in reperfusion, as opposed to sole platelet application. Known blockers of endogenously released anti-platelet substances like nitric oxide, PGI(2) or adenosine did not mediate a further aggravation of myocardial dysfunction. The platelet-activating factor (PAF) antagonist WEB 2170 BS, however, significantly improved recovery of external heart work during ischemia and in reperfusion. This indicates that an additive cardiodepressive effect of PMN and platelets in working guinea pig hearts exposed to a low-flow ischemia, cannot be demonstrated, whereas PAF antagonists seem to be cardioprotective, under these conditions. Even addition of fibrinogen to the perfusate, did not show an additive cardiodepressive effect of coapplication of PMN and platelets.

• Authors: Christian Seligmann, Tobias Leitsch, Magda Kusus, Andreas Bock, Mike Schimmer, Yusuf Simsek, Werner G Daniel
• Journal: Journal of vascular research (J Vasc Res) 2003 Nov-Dec Vol. 40 Issue 6 Pg. 501-8 ISSN: 1018-1172 [Print] Switzerland
• PMID: 14631104 (Publication Type: Journal Article)
• Copyright: Copyright 2003 S. Karger AG, Basel

Chemical References

• Reactive Oxygen Species
• Fibrinogen

Topics

• Animals
• Blood Platelets (metabolism)
• Cell Adhesion (drug effects, immunology)
• Fibrinogen (pharmacology)
• Guinea Pigs
• In Vitro Techniques
• Myocardial Contraction
• Myocardial Reperfusion Injury (metabolism, pathology)
• Myocardium (metabolism, pathology)
• Neutrophils (metabolism)
• Platelet Adhesiveness
• Reactive Oxygen Species (metabolism)