Abstract | OBJECTIVE: METHODS: RESULTS: Serum samples were tested by ELISA using SEA treated with sodium periodate (SP-SEA-ELISA), including 64 sera from cases with chronic schistosomiasis japonica, 119 sera from normal individuals in non-endemic area, 34 sera from patients with clonorchiasis, 33 sera of paragonimiasis cases and 36 sera from patients with cysticercosis. The results showed that its sensitivity (98.4%) was similar to that of the routine SEA-ELISA (100.0%) (P > 0.05) and the specificity is higher than that of the SEA-ELISA (P < 0.05). SP-SEA-ELISA showed a higher negative rate (89.0%) for sera of schistosomiasis patients 12 months post-treatment than that of the SEA-ELISA (42.1%). CONCLUSION: Use of SP-SEA can increase the specificity of ELISA, reduce cross-reactivity with serum samples from cases infected with other parasites and improve its value in evaluating therapeutic efficacy.
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Authors | Yue-long Huang, Xin-yuan Yi, Xian-fang Zeng, Ran Zhang, Shi-shan Yuan |
Journal | Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
(Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi)
Vol. 21
Issue 4
Pg. 238-41
( 2003)
ISSN: 1000-7423 [Print] China |
PMID | 14628379
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Helminth
- Antigens, Helminth
- Periodic Acid
- metaperiodate
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Topics |
- Animals
- Antibodies, Helminth
(blood)
- Antibody Specificity
- Antigens, Helminth
- Enzyme-Linked Immunosorbent Assay
- Humans
- Periodic Acid
- Rabbits
- Schistosoma japonicum
(immunology)
- Schistosomiasis japonica
(diagnosis)
- Sensitivity and Specificity
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