Abstract | SETTING:
Bovine tuberculosis is a problem in a number of countries and protection of cattle by vaccination could be an important control strategy. OBJECTIVES: DESIGN: Groups of cattle (n=10) were vaccinated with MPB83 DNA, MPB70 DNA, or MPB70 DNA followed by MPB70 protein or injected with BCG or control plasmid DNA. Animals were challenged intratracheally with virulent Mycobacterium bovis at 13 weeks and protection assessed 17 weeks later at postmortem. RESULTS: In contrast to the strong cellular immune responses induced by BCG, the DNA vaccines induced minimal interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) responses. Cattle primed with MPB70 DNA and boosted with MPB70 protein induced a strong antibody response and a weak IFN-gamma response. BCG gave significant reduction in four pathological parameters of disease while the DNA vaccines and MPB70 DNA/ protein did not protect animals against challenge with M. bovis. Moreover, cattle vaccinated with MPB70 DNA/ protein had a significantly higher proportion of animals with severe lung lesions (>100 lesions) than the MPB70 DNA alone or the control group. Increased bovine PPD-specific IL-4 mRNA expression in cattle, post-challenge, correlated with the presence of tuberculous lung lesions. CONCLUSION:
|
Authors | D N Wedlock, M A Skinner, N A Parlane, H M Vordermeier, R G Hewinson, G W de Lisle, B M Buddle |
Journal | Tuberculosis (Edinburgh, Scotland)
(Tuberculosis (Edinb))
Vol. 83
Issue 6
Pg. 339-49
( 2003)
ISSN: 1472-9792 [Print] Scotland |
PMID | 14623164
(Publication Type: Clinical Trial, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antigens, Bacterial
- BCG Vaccine
- Bacterial Proteins
- Interleukin-2
- MPB83 protein, Mycobacterium
- Membrane Proteins
- Tuberculosis Vaccines
- Vaccines, DNA
- MPB70 protein, Mycobacterium bovis
- Interleukin-4
- Interferon-gamma
|
Topics |
- Animals
- Antigens, Bacterial
(immunology)
- BCG Vaccine
(administration & dosage, immunology)
- Bacterial Proteins
(immunology)
- Cattle
- Interferon-gamma
(blood)
- Interleukin-2
(blood)
- Interleukin-4
(blood)
- Membrane Proteins
(immunology)
- Mycobacterium bovis
(immunology)
- Severity of Illness Index
- T-Lymphocytes
(immunology)
- Tuberculosis Vaccines
(administration & dosage, immunology)
- Tuberculosis, Bovine
(immunology, pathology, prevention & control)
- Tuberculosis, Pulmonary
(immunology, pathology, veterinary)
- Vaccines, DNA
(administration & dosage, immunology)
|