Abstract |
The immune responses induced against Leishmania antigens in volunteers who were vaccinated in a double-blind, randomized field efficacy trial of a preparation of autoclaved Leishmania major (ALM) mixed with a low dose of Bacille Calmette-Guerin vaccine (BCG) who developed either a cutaneous leishmaniasis (CL) lesion due to exposure to infected sandfly bite(s) or did not develop a lesion during the course of the trial were studied and compared with those of non-vaccinated controls. Blood samples were also assayed from different groups including volunteers with history of CL and volunteers with previous positive or negative leishmanin skin test (LST) without a history of CL. The vaccinated volunteers had received a single dose of either ALM mixed with a low dose of BCG or the same dose of BCG alone. The LST and in vitro proliferative response (stimulation index, SI), interferon gamma (IFN-gamma) production and, in a few cases, interleukin (IL)-4 production of peripheral blood mononuclear cells to soluble Leishmania antigens were measured. The results indicated that volunteers who developed CL in the vaccine arm showed a slightly higher SI than cases who received BCG alone. Volunteers with history of CL and volunteers with positive LST demonstrated the strongest proliferation indices and IFN-gamma production. The data suggest that a single dose of ALM + BCG induces a weak Th1 response in vaccinated volunteers that is far lower than that in volunteers with prior subclinical infection or volunteers with history of CL, who are presumed to be immune.
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Authors | M Mahmoodi, A Khamesipour, Y Dowlati, S Rafati, A Z Momeni, M Emamjomeh, H Hejazi, F Modabber |
Journal | Clinical and experimental immunology
(Clin Exp Immunol)
Vol. 134
Issue 2
Pg. 303-8
(Nov 2003)
ISSN: 0009-9104 [Print] England |
PMID | 14616791
(Publication Type: Clinical Trial, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Protozoan
- BCG Vaccine
- Leishmaniasis Vaccines
- Protozoan Vaccines
- Vaccines, Combined
- autoclaved Leishmania major vaccine
- Interleukin-4
- Interferon-gamma
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Topics |
- Adolescent
- Adult
- Animals
- Antigens, Protozoan
(immunology)
- BCG Vaccine
(immunology)
- Cell Division
(immunology)
- Cells, Cultured
- Double-Blind Method
- Humans
- Interferon-gamma
(biosynthesis)
- Interleukin-4
(biosynthesis)
- Leishmania major
(immunology)
- Leishmaniasis Vaccines
- Leishmaniasis, Cutaneous
(immunology, prevention & control)
- Lymphocyte Activation
- Middle Aged
- Protozoan Vaccines
(immunology)
- Th1 Cells
(immunology)
- Vaccines, Combined
(immunology)
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