A neuroprotective effect of adenosine A1-receptor agonists on ischemia-induced decrease in 2-deoxyglucose uptake in rat hippocampal slices.

Abstract

The effects of adenosine (A) receptor agonists on ischemia-induced impairment of 2-deoxyglucose (2-DG) uptake by rat hippocampal slices was evaluated. Hippocampal slices were exposed to 20-min hypoxia + hypoglycemia (ischemia) and then returned to oxygenated and glucose-containing Krebs-Ringer solution for 6 h. Ischemia reduced 2-DG uptake in the hippocampal slices. The ischemia-induced reduction in 2-DG uptake was attenuated by pretreatment with A1 receptor agonists but not with A2 receptor agonists. 8-Phenyltheophylline, an A1 receptor antagonist, exacerbated the ischemia-induced decrease. The A1 receptor agonist-induced neuroprotective effect was blocked by co-treatment with 8-phenyltheophylline. The present study suggests that the A1 receptor-mediated function has a protective role in ischemia-induced decreases in glucose metabolism in hippocampal slices.

• Authors: K Tominaga, S Shibata, S Watanabe
• Journal: Neuroscience letters (Neurosci Lett) Vol. 145 Issue 1 Pg. 67-70 (Sep 28 1992) ISSN: 0304-3940 [Print] Ireland
• PMID: 1461571 (Publication Type: Journal Article)

Chemical References

• Purinergic Antagonists
• Receptors, Purinergic
• Vasodilator Agents
• Phenylisopropyladenosine
• Adenosine-5'-(N-ethylcarboxamide)
• N(6)-cyclohexyladenosine
• Deoxyglucose
• Theophylline
• 8-phenyltheophylline
• Adenosine

Topics

• Adenosine (analogs & derivatives, pharmacology)
• Adenosine-5'-(N-ethylcarboxamide)
• Animals
• Brain Ischemia (metabolism)
• Deoxyglucose (metabolism)
• Hippocampus (metabolism)
• In Vitro Techniques
• Male
• Nervous System Diseases (chemically induced, physiopathology)
• Phenylisopropyladenosine (pharmacology)
• Purinergic Antagonists
• Rats
• Rats, Wistar
• Receptors, Purinergic (physiology)
• Theophylline (analogs & derivatives, pharmacology)
• Vasodilator Agents (pharmacology)