Expression of gicerin enhances the invasive and metastatic activities of a mouse mammary carcinoma cell line.

Abstract	Gicerin, an immunoglobulin superfamily protein, is expressed abundantly in the embryonic tissues and plays an important role in development through its cell adhesive activity. Interestingly, re-expression of gicerin is found in a variety of tumors. In the present study, the possible role of gicerin in the progression of mammary carcinoma was investigated. The normal mammary glands of mice were negative for gicerin, but sporadic mammary carcinoma cells expressed gicerin strongly on their surface. A mouse mammary carcinoma cell line, JYG-B, which is gicerin-negative was employed for introducing gicerin cDNA and the resultant gicerin transfectants were subsequently analyzed. In vitro, self-aggregation activity of the gicerin-transfectants progressed. For an in vivo study, invasive and metastatic potential of the cells was examined by a subcutaneous implantation into nude mice. The invasion of gicerin transfectants into the surrounding tissue was enhanced and severe metastasis to the lungs occurred. These findings suggest that gicerin is an effector for the malignant progression of mammary carcinoma.
Authors	Yasuhiro Tsukamoto, Takashi Sakaiuchi, Satoshi Hiroi, Masaru Furuya, Seiichiro Tsuchiya, Fumihiko Sasaki, Naomasa Miki, Eiichi Taira
Journal	International journal of oncology (Int J Oncol) Vol. 23 Issue 6 Pg. 1671-7 (Dec 2003) ISSN: 1019-6439 [Print] Greece
PMID	14612940 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Avian Proteins CD146 Antigen Carrier Proteins Cell Adhesion Molecules DNA, Complementary Mcam protein, mouse Nerve Tissue Proteins
Topics	Animals Avian Proteins Blotting, Western CD146 Antigen Carrier Proteins (biosynthesis, metabolism) Cell Adhesion Molecules (biosynthesis, metabolism) Cell Line, Tumor Cell Membrane (metabolism) DNA, Complementary (metabolism) Disease Progression Female Immunohistochemistry Lung Neoplasms (pathology) Mammary Glands, Animal (metabolism) Mammary Neoplasms, Animal (pathology) Mice Mice, Inbred BALB C Mice, Nude Neoplasm Invasiveness Neoplasm Metastasis Neoplasm Transplantation Nerve Tissue Proteins (biosynthesis) Time Factors Transfection

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!