Abstract |
Factors such as charge, aggregation and lipophilicity influence photosensitiser localisation. The lipophilic octasubstituted sensitiser 2,3,9,10,16,17,23,24-octakis(decyloxy)phthalocyaninato zinc(II) was incorporated into liposomes of dimyristoyl-L-alpha- phosphatidylcholine ( DMPC), non-ionic micelles of Tween 80 and the hydrosoluble polymer Solutol HS 15 in order to investigate how these different environments affect the photophysical properties and phototoxicity of the photosensitiser. Fluorescence quantum yields and singlet molecular oxygen generation are enhanced in the presence of Solutol HS 15. Phototoxicities were calculated by employing a concentration of 10(-7) M of the dye against the Hep-2 cell line, which showed a viability of 53 and 30% in DMPC and Solutol HS 15, respectively. After 24 h of photodynamic therapy with 15 min irradiation, apoptotic and necrotic cells were observed.
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Authors | Myriam E Rodriguez, Flavia Morán, Analia Bonansea, Marisa Monetti, Daniel A Fernández, Cristian A Strassert, Viviana Rivarola, Josefina Awruch, Lelia E Dicelio |
Journal | Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
(Photochem Photobiol Sci)
Vol. 2
Issue 10
Pg. 988-94
(Oct 2003)
ISSN: 1474-905X [Print] England |
PMID | 14606753
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Liposomes
- Micelles
- Organometallic Compounds
- Photosensitizing Agents
- Polymers
- Dimyristoylphosphatidylcholine
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Topics |
- Apoptosis
(drug effects)
- Cell Line
- Cell Survival
(drug effects)
- Dimyristoylphosphatidylcholine
- Humans
- Liposomes
- Micelles
- Necrosis
- Organometallic Compounds
(chemistry, toxicity)
- Photobiology
- Photochemistry
- Photochemotherapy
- Photosensitizing Agents
(chemistry, toxicity)
- Polymers
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